High-Throughput Silencing Using the CRISPR-Cas9 System: A Review of the Benefits and Challenges (Q38498286)

3 September 2017

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title

High-Throughput Silencing Using the CRISPR-Cas9 System: A Review of the Benefits and Challenges (English)

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3 September 2017

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Mark Wade

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3 September 2017

publication date

22 May 2015

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Journal of Biomolecular Screening

3 September 2017

published in

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3 September 2017

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3 September 2017

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3 September 2017

page(s)

1027-1039

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CRISPR-based adaptive and heritable immunity in prokaryotes

3 September 2017

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Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression

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CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes

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A protein-tagging system for signal amplification in gene expression and fluorescence imaging

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In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.

21 January 2018

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Improved genome editing in human cell lines using the CRISPR method

21 January 2018

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An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo

21 January 2018

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Inducible in vivo genome editing with CRISPR-Cas9

21 January 2018

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Single-cell RNA-seq reveals dynamic, random monoallelic gene expression in mammalian cells

21 January 2018

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Haploid genetic screens in human cells identify host factors used by pathogens

21 January 2018

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Generation of iPSCs from cultured human malignant cells

21 January 2018

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Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line

21 January 2018

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Forward and reverse genetics through derivation of haploid mouse embryonic stem cells.

21 January 2018

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Derivation of haploid embryonic stem cells from mouse embryos

21 January 2018

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https://api.crossref.org/works/10.1177%2F1087057115587916

A haploid genetic screen identifies the major facilitator domain containing 2A (MFSD2A) transporter as a key mediator in the response to tunicamycin

21 January 2018

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https://api.crossref.org/works/10.1177%2F1087057115587916

A genetic screen using the PiggyBac transposon in haploid cells identifies Parp1 as a mediator of olaparib toxicity

21 January 2018

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https://api.crossref.org/works/10.1177%2F1087057115587916

Inhibition of ATPIF1 ameliorates severe mitochondrial respiratory chain dysfunction in mammalian cells.

21 January 2018

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https://api.crossref.org/works/10.1177%2F1087057115587916

Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors.

21 January 2018

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https://api.crossref.org/works/10.1177%2F1087057115587916

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

21 January 2018

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https://api.crossref.org/works/10.1177%2F1087057115587916

Locked nucleic acid (LNA) mediated improvements in siRNA stability and functionality

21 January 2018

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https://api.crossref.org/works/10.1177%2F1087057115587916

Position-specific chemical modification of siRNAs reduces "off-target" transcript silencing

21 January 2018

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https://api.crossref.org/works/10.1177%2F1087057115587916

Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity

21 January 2018

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Selection of hyperfunctional siRNAs with improved potency and specificity

21 January 2018

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Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application

21 January 2018

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Widespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarity

21 January 2018

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Discovery of a dicer-independent, cell-type dependent alternate targeting sequence generator: implications in gene silencing & pooled RNAi screens

21 January 2018

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Systematic analysis of RNAi reports identifies dismal commonality at gene-level and reveals an unprecedented enrichment in pooled shRNA screens

21 January 2018

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Comparative analysis of RNAi screening technologies at genome-scale reveals an inherent processing inefficiency of the plasmid-based shRNA hairpin

21 January 2018

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High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

21 January 2018

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Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

21 January 2018

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Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

21 January 2018

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Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

21 January 2018

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Gene targeting with viral vectors

21 January 2018

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https://api.crossref.org/works/10.1177%2F1087057115587916

Adeno-associated virus biology

21 January 2018

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https://api.crossref.org/works/10.1177%2F1087057115587916

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

21 January 2018

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https://api.crossref.org/works/10.1177%2F1087057115587916

Targeted genome regulation and modification using transcription activator-like effectors

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Homology-directed repair of DNA nicks via pathways distinct from canonical double-strand break repair

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Conditional targeting of Ispd using paired Cas9 nickase and a single DNA template in mice

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Human gene targeting by viral vectors

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Human Gene Targeting by Adeno-Associated Virus Vectors Is Enhanced by DNA Double-Strand Breaks

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Simple monitoring of gene targeting efficiency in human somatic cell lines using the PIGA gene

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Single-strand nicks induce homologous recombination with less toxicity than double-strand breaks using an AAV vector template

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

CRISPR genome engineering and viral gene delivery: a case of mutual attraction

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

CRISPR/Cas9-mediated genome engineering: an adeno-associated viral (AAV) vector toolbox

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Rapid modelling of cooperating genetic events in cancer through somatic genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Correction of a genetic disease in mouse via use of CRISPR-Cas9.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

DrugTargetSeqR: a genomics- and CRISPR-Cas9-based method to analyze drug targets

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

DNA sequencing and CRISPR-Cas9 gene editing for target validation in mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Understanding functional miRNA-target interactions in vivo by site-specific genome engineering.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Chromosomal translocations in human cells are generated by canonical nonhomologous end-joining.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Precise correction of the dystrophin gene in duchenne muscular dystrophy patient induced pluripotent stem cells by TALEN and CRISPR-Cas9.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Identifying drug-target selectivity of small-molecule CRM1/XPO1 inhibitors by CRISPR/Cas9 genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations that cause Duchenne muscular dystrophy.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Mapping of signaling networks through synthetic genetic interaction analysis by RNAi

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Measuring genetic interactions in human cells by RNAi and imaging

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Manipulating mitochondrial DNA heteroplasmy by a mitochondrially targeted restriction endonuclease

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Specific elimination of mutant mitochondrial genomes in patient-derived cells by mitoTALENs

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Efficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

The multilayered complexity of ceRNA crosstalk and competition

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Circular RNAs: diversity of form and function

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Programmable RNA recognition and cleavage by CRISPR/Cas9.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

DNA targeting specificity of RNA-guided Cas9 nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Target specificity of the CRISPR-Cas9 system.

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

A genome-scale RNA interference screen implicates NF1 loss in resistance to RAF inhibition

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

A melanocyte lineage program confers resistance to MAP kinase pathway inhibition

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe

21 January 2018

1 reference

https://api.crossref.org/works/10.1177%2F1087057115587916

21 January 2018

Identifiers

DOI

10.1177/1087057115587916

1 reference

3 September 2017

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