Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification (Q29029259)

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scientific article (publication date: 25 April 2014)

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English	Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification	scientific article (publication date: 25 April 2014)

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Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification (English)

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https://doi.org/10.1038%2FNBT.2909

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26 March 2017

main subject

Cas9

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author name string

John P Guilinger

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1

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https://doi.org/10.1038%2FNBT.2909

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26 March 2017

David B Thompson

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https://doi.org/10.1038%2FNBT.2909

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26 March 2017

David R Liu

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3

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https://doi.org/10.1038%2FNBT.2909

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26 March 2017

publication date

25 April 2014

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https://doi.org/10.1038%2FNBT.2909

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26 March 2017

published in

Nature Biotechnology

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https://doi.org/10.1038%2FNBT.2909

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26 March 2017

volume

32

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https://doi.org/10.1038%2FNBT.2909

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issue

6

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https://doi.org/10.1038%2FNBT.2909

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26 March 2017

page(s)

577-582

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https://doi.org/10.1038%2FNBT.2909

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26 March 2017

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ImageJ

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Europe PubMed Central

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11 June 2022

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https://www.ebi.ac.uk/europepmc/webservices/rest/PMC4263420/fullTextXML

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26 March 2017

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Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

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Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

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7 April 2017

Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases

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DNA targeting specificity of RNA-guided Cas9 nucleases

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Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

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Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity

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Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

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Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

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In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites

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High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

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Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects

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CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity

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A recombinant polypeptide extends the in vivo half-life of peptides and proteins in a tunable manner

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TALENs and ZFNs are associated with different mutation signatures

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FokI requires two specific DNA sites for cleavage

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reference URL

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A rapid and general assay for monitoring endogenous gene modification

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Crossref

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Identifiers

DOI

10.1038/NBT.2909

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DOI.org query endpoint

reference URL

https://doi.org/10.1038%2FNBT.2909

retrieved

26 March 2017

stated in

Consolidated OpenCitations Corpus – April 2017

OpenCitations bibliographic resource ID

247041

Dimensions Publication ID

1053226125

0 references

OpenCitations bibliographic resource ID

247041

1 reference

stated in

Consolidated OpenCitations Corpus – April 2017

OpenCitations bibliographic resource ID

247041

PMC publication ID

4263420

1 reference

stated in

Consolidated OpenCitations Corpus – April 2017

OpenCitations bibliographic resource ID

247041

PubMed publication ID

24770324

1 reference

stated in

Consolidated OpenCitations Corpus – April 2017

OpenCitations bibliographic resource ID

247041

 

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