Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool (Q43125635)

14 November 2017

Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool (English)

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14 November 2017

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Lasse Ebdrup Pedersen

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Thomas Beuchert Kallehauge

14 November 2017

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14 November 2017

Alex Toftgaard Nielsen

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Helene Faustrup Kildegaard

14 November 2017

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14 November 2017

Carlotta Ronda

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Henning Gram Hansen

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Michael J Betenbaugh

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14 November 2017

22 May 2014

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Biotechnology and Bioengineering

14 November 2017

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14 November 2017

111

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14 November 2017

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14 November 2017

1604-1616

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describes a project that uses

14 November 2017

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1 reference

11 June 2022

https://www.ebi.ac.uk/europepmc/webservices/rest/PMC4312910/fullTextXML

based on heuristic

inferred from PubMed Central ID database lookup

cites work

Genetic screens in human cells using the CRISPR-Cas9 system

1 reference

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A guide RNA sequence design platform for the CRISPR/Cas9 system for model organism genomes.

25 May 2018

1 reference

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Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

25 May 2018

1 reference

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One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering

25 May 2018

1 reference

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CRISPR/Cas9-targeted mutagenesis in Caenorhabditis elegans

25 May 2018

1 reference

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Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome

25 May 2018

1 reference

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DNA targeting specificity of RNA-guided Cas9 nucleases

25 May 2018

1 reference

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Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system.

25 May 2018

1 reference

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High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

25 May 2018

1 reference

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Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.

25 May 2018

1 reference

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One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering

25 May 2018

1 reference

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Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos

25 May 2018

1 reference

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The emerging CHO systems biology era: harnessing the 'omics revolution for biotechnology

25 May 2018

1 reference

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Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

25 May 2018

1 reference

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RNA-programmed genome editing in human cells

25 May 2018

1 reference

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Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

25 May 2018

1 reference

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Efficient genome editing in zebrafish using a CRISPR-Cas system

25 May 2018

1 reference

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RNA-guided human genome engineering via Cas9

25 May 2018

1 reference

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Multiplex genome engineering using CRISPR/Cas systems

25 May 2018

1 reference

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A CRISPR approach to gene targeting

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

25 May 2018

1 reference

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Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction

25 May 2018

1 reference

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Chinese hamster genome database: an online resource for the CHO community at www.CHOgenome.org

25 May 2018

1 reference

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Mining the O-glycoproteome using zinc-finger nuclease-glycoengineered SimpleCell lines

25 May 2018

1 reference

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Adaptive evolution of drug targets in producer and non-producer organisms

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

A TALE nuclease architecture for efficient genome editing

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

Highly efficient deletion of FUT8 in CHO cell lines using zinc-finger nucleases yields cells that produce completely nonfucosylated antibodies.

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

Cosmc is an essential chaperone for correct protein O-glycosylation

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

Targeted approaches for gene therapy and the emergence of engineered meganucleases

25 May 2018

1 reference

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Biopython: freely available Python tools for computational molecular biology and bioinformatics

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases.

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments.

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

Establishment of FUT8 knockout Chinese hamster ovary cells: an ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type oligosaccharides shows the critical role of enhancing antibody-dependent cellular cytotoxicity

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicity

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

The alpha1-6-fucosyltransferase gene and its biological significance

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

Positive genetic selection for gene disruption in mammalian cells by homologous recombination

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

BAK and BAX deletion using zinc-finger nucleases yields apoptosis-resistant CHO cells

15 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

Engineering Chinese hamster ovary cells to maximize effector function of produced antibodies using FUT8 siRNA.

15 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

Defucosylated chimeric anti-CC chemokine receptor 4 IgG1 with enhanced antibody-dependent cellular cytotoxicity shows potent therapeutic activity to T-cell leukemia and lymphoma

15 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4312910

The control of glycoprotein synthesis: N-acetylglucosamine linkage to a mannose residue as a signal for the attachment of L-fucose to the asparagine-linked N-acetylglucosamine residue of glycopeptide from alpha1-acid glycoprotein

15 August 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/24827782

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1002/BIT.25233

1 reference

14 November 2017

1 reference

14 November 2017

PubMed publication ID

24827782

1 reference