Methods for the directed evolution of proteins (Q38521229)

3 September 2017

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title

Methods for the directed evolution of proteins (English)

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3 September 2017

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Michael S Packer

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3 September 2017

David R Liu

series ordinal

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3 September 2017

publication date

9 June 2015

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3 September 2017

published in

Nature Reviews Genetics

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3 September 2017

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3 September 2017

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3 September 2017

page(s)

379-394

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The effects of artificial selection on the maize genome

3 September 2017

cites work

1 reference

From wild animals to domestic pets, an evolutionary view of domestication

21 January 2018

1 reference

Diversifying carotenoid biosynthetic pathways by directed evolution

21 January 2018

1 reference

Directed evolution of Methanococcus jannaschii citramalate synthase for biosynthesis of 1-propanol and 1-butanol by Escherichia coli

21 January 2018

1 reference

Programming cells by multiplex genome engineering and accelerated evolution

21 January 2018

1 reference

Genome shuffling leads to rapid phenotypic improvement in bacteria

21 January 2018

1 reference

Engineering yeast transcription machinery for improved ethanol tolerance and production.

21 January 2018

1 reference

Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes

21 January 2018

1 reference

Directed evolution of a far-red fluorescent rhodopsin

21 January 2018

1 reference

Guiding the Selection of Human Antibodies from Phage Display Repertoires to a Single Epitope of an Antigen

21 January 2018

1 reference

A new approach to random mutagenesis in vitro

21 January 2018

1 reference

A general method for saturation mutagenesis of cloned DNA fragments

21 January 2018

1 reference

The specific mutagenic effect of base analogues on Phage T4

21 January 2018

1 reference

Mutagenic repair in Escherichia coli: products of the recA gene and of the umuD and umuC genes act at different steps in UV-induced mutagenesis

21 January 2018

1 reference

Bacterial mutator genes and the control of spontaneous mutation

21 January 2018

1 reference

An efficient random mutagenesis technique using anE.coli mutator strain

21 January 2018

1 reference

Identification of the epsilon-subunit of Escherichia coli DNA polymerase III holoenzyme as the dnaQ gene product: a fidelity subunit for DNA replication

21 January 2018

1 reference

An orthogonal DNA replication system in yeast

21 January 2018

1 reference

An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues

21 January 2018

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High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase

21 January 2018

1 reference

Directed enzyme evolution via small and effective neutral drift libraries

21 January 2018

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Reducing mutational bias in random protein libraries

21 January 2018

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Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution

21 January 2018

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Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

21 January 2018

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Enzymatic assembly of DNA molecules up to several hundred kilobases

21 January 2018

1 reference

Circular polymerase extension cloning of complex gene libraries and pathways

21 January 2018

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USER Cloning and USER Fusion: The Ideal Cloning Techniques for Small and Big Laboratories

21 January 2018

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Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences

21 January 2018

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The consensus concept for thermostability engineering of proteins

21 January 2018

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Reconstructed evolutionary adaptive paths give polymerases accepting reversible terminators for sequencing and SNP detection

21 January 2018

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Engineering V-type nerve agents detoxifying enzymes using computationally focused libraries

21 January 2018

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Macromolecular modeling with rosetta

21 January 2018

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Computationally designed libraries for rapid enzyme stabilization

21 January 2018

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Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries

21 January 2018

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Rapid evolution of a protein in vitro by DNA shuffling

21 January 2018

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DNA shuffling method for generating highly recombined genes and evolved enzymes

21 January 2018

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Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolution

21 January 2018

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Molecular evolution by staggered extension process (StEP) in vitro recombination

21 January 2018

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Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

21 January 2018

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Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently

21 January 2018

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Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution

21 January 2018

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Improved green fluorescent protein by molecular evolution using DNA shuffling

21 January 2018

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DNA shuffling of a family of genes from diverse species accelerates directed evolution

21 January 2018

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A Heritable Recombination system for synthetic Darwinian evolution in yeast

21 January 2018

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Libraries of hybrid proteins from distantly related sequences

21 January 2018

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A combinatorial approach to hybrid enzymes independent of DNA homology

21 January 2018

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Directed evolution of protein enzymes using nonhomologous random recombination

21 January 2018

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Protein building blocks preserved by recombination

21 January 2018

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General method for sequence-independent site-directed chimeragenesis

21 January 2018

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Directed evolution of proteins by exon shuffling

21 January 2018

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Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

21 January 2018

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Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide

21 January 2018

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Wavelength mutations and posttranslational autoxidation of green fluorescent protein

21 January 2018

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Optical recording of action potentials in mammalian neurons using a microbial rhodopsin

21 January 2018

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Luminogenic cytochrome P450 assays

21 January 2018

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A high-throughput cellulase screening system based on droplet microfluidics

21 January 2018

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Evolution of a designed retro-aldolase leads to complete active site remodeling

21 January 2018

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Enzyme assays for high-throughput screening

21 January 2018

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A novel genetic system to detect protein-protein interactions

21 January 2018

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Chemical complementation: a reaction-independent genetic assay for enzyme catalysis.

21 January 2018

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Directed evolution of a glycosynthase via chemical complementation.

21 January 2018

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High-throughput selection for cellulase catalysts using chemical complementation

21 January 2018

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Targeted mutagenesis of the Vibrio fischeri flavin reductase FRase I to improve activation of the anticancer prodrug CB1954.

21 January 2018

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Correlation between catalytic efficiency and the transcription read-out in chemical complementation: a general assay for enzyme catalysis

21 January 2018

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Electronic Separation of Biological Cells by Volume

21 January 2018

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Yeast surface display for screening combinatorial polypeptide libraries

21 January 2018

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Directed evolution of the site specificity of Cre recombinase

21 January 2018

1 reference

Directed evolution of a small-molecule-triggered intein with improved splicing properties in mammalian cells

21 January 2018

1 reference

A general method for greatly improving the affinity of antibodies by using combinatorial libraries

21 January 2018

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Mining a yeast library for brain endothelial cell-binding antibodies

21 January 2018

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A general strategy for the evolution of bond-forming enzymes using yeast display

21 January 2018

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Immobilization of actively thromboresistant assemblies on sterile blood-contacting surfaces

21 January 2018

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Engineered red blood cells as carriers for systemic delivery of a wide array of functional probes

21 January 2018

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Protein thioester synthesis enabled by sortase

21 January 2018

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Receptor-directed chimeric toxins created by sortase-mediated protein fusion

21 January 2018

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Flow-based enzymatic ligation by sortase A.

21 January 2018

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Reprogramming the specificity of sortase enzymes.

21 January 2018

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Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries

21 January 2018

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Man-made cell-like compartments for molecular evolution

21 January 2018

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In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting

21 January 2018

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Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

21 January 2018

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Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells

21 January 2018

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Evolution of enzyme catalysts caged in biomimetic gel-shell beads

21 January 2018

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Rapid isolation of high-affinity protein binding peptides using bacterial display

21 January 2018

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Phage antibodies: filamentous phage displaying antibody variable domains

21 January 2018

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Making antibody fragments using phage display libraries

21 January 2018

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Searching for Peptide Ligands with an Epitope Library

21 January 2018

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High efficiency transformation of E. coli by high voltage electroporation

21 January 2018

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In vitro selection and evolution of functional proteins by using ribosome display

21 January 2018

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The use of mRNA display to select high-affinity protein-binding peptides

21 January 2018

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In vitro selection for catalytic activity with ribosome display

21 January 2018

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Selection and evolution of enzymes from a partially randomized non-catalytic scaffold

21 January 2018

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Predicting the emergence of antibiotic resistance by directed evolution and structural analysis

21 January 2018

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Understanding, predicting and manipulating the genotypic evolution of antibiotic resistance.

21 January 2018

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Engineering a tRNA and aminoacyl-tRNA synthetase for the site-specific incorporation of unnatural amino acids into proteins in vivo

21 January 2018

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An efficient system for the evolution of aminoacyl-tRNA synthetase specificity

21 January 2018

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Structure-guided reprogramming of serine recombinase DNA sequence specificity

21 January 2018

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Rewiring yeast sugar transporter preference through modifying a conserved protein motif

21 January 2018

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Directed evolution of xylose isomerase for improved xylose catabolism and fermentation in the yeast Saccharomyces cerevisiae

21 January 2018

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Directed evolution of a protein container

21 January 2018

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Redesign of extensive protein-DNA interfaces of meganucleases using iterative cycles of in vitro compartmentalization

21 January 2018

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Directed evolution of polymerase function by compartmentalized self-replication

21 January 2018

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CyDNA: synthesis and replication of highly Cy-dye substituted DNA by an evolved polymerase.

21 January 2018

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Molecular breeding of polymerases for amplification of ancient DNA

21 January 2018

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Directed evolution of genetic parts and circuits by compartmentalized partnered replication

21 January 2018

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In vivo continuous directed evolution

21 January 2018

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Dynamics of adaptation and diversification: a 10,000-generation experiment with bacterial populations

21 January 2018

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Evolutionary paths to antibiotic resistance under dynamically sustained drug selection

21 January 2018

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Directed evolution of a model primordial enzyme provides insights into the development of the genetic code

21 January 2018

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Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I.

21 January 2018

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Adaptive molecular evolution for 13,000 phage generations: a possible arms race

21 January 2018

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A system for the continuous directed evolution of biomolecules

21 January 2018

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Negative selection and stringency modulation in phage-assisted continuous evolution

21 January 2018

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A system for the continuous directed evolution of proteases rapidly reveals drug-resistance mutations

21 January 2018

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De novo computational design of retro-aldol enzymes

21 January 2018

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Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction

21 January 2018

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De novo design of a transmembrane Zn²⁺-transporting four-helix bundle

21 January 2018

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Accurate design of co-assembling multi-component protein nanomaterials

21 January 2018

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A designed supramolecular protein assembly with in vivo enzymatic activity

21 January 2018

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A De Novo Protein Binding Pair By Computational Design and Directed Evolution

21 January 2018

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Directed evolution of the peroxidase activity of a de novo-designed protein

21 January 2018

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Optimization of the In-Silico-Designed Kemp Eliminase KE70 by Computational Design and Directed Evolution

21 January 2018

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Kemp elimination catalysts by computational enzyme design

21 January 2018

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Biocatalytic asymmetric synthesis of chiral amines from ketones applied to sitagliptin manufacture

21 January 2018

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Novel methods for directed evolution of enzymes: quality, not quantity

21 January 2018

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Single-cell high-throughput screening to identify enantioselective hydrolytic enzymes.

21 January 2018

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Selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display.

21 January 2018

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Directed evolution of sortase A mutants with altered substrate selectivity profiles

21 January 2018

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Directed evolution of hydrolases for prevention of G-type nerve agent intoxication

21 January 2018

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Directed evolution of substrate-optimized GroEL/S chaperonins

21 January 2018

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One-step enzymatic modification of the cell surface redirects cellular cytotoxicity and parasite tropism

31 March 2020

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High-efficiency transformation of yeast by electroporation

31 March 2020

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Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase Variants for in Vivo or in Vitro Synthetic Circuitry

31 March 2020

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Exceptional convergent evolution in a virus.

31 March 2020

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/26055155

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Randomization of genes by PCR mutagenesis

1 reference

inferred from DOI database lookup

7 January 2021

based on heuristic

Identifiers

DOI

10.1038/NRG3927

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Dimensions Publication ID

3 September 2017

1048146186

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