Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolution (Q24816006)

4 July 2017

title

Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolution (English)

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4 July 2017

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Kristian M Müller

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4 July 2017

Sabine C Stebel

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4 July 2017

Susanne Knall

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4 July 2017

Gregor Zipf

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4 July 2017

Hubert S Bernauer

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4 July 2017

Katja M Arndt

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4 July 2017

language of work or name

English

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publication date

1 August 2005

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4 July 2017

published in

Nucleic Acids Research

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4 July 2017

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4 July 2017

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4 July 2017

page(s)

e117

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DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

4 July 2017

cites work

1 reference

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57 Sequencing end-labeled DNA with base-specific chemical cleavages

7 April 2017

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Rapid evolution of a protein in vitro by DNA shuffling

7 April 2017

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Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution

7 April 2017

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A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. I. PURIFICATION OF THE ENZYME AND CHARACTERIZATION OF THE PHOSPHATASE ACTIVITY

28 September 2017

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Random DNA fragmentation with endonuclease V: application to DNA shuffling

28 September 2017

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Libraries of hybrid proteins from distantly related sequences

28 September 2017

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DNA shuffling method for generating highly recombined genes and evolved enzymes

28 September 2017

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Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides.

28 September 2017

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Multiple amino acid substitutions allow DNA polymerases to synthesize RNA.

28 September 2017

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Role of electrophilic and general base catalysis in the mechanism of Escherichia coli uracil DNA glycosylase.

28 September 2017

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Molecular evolution by staggered extension process (StEP) in vitro recombination

28 September 2017

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Random-priming in vitro recombination: an effective tool for directed evolution

28 September 2017

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Optimization of DNA shuffling for high fidelity recombination

28 September 2017

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Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities

28 September 2017

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Evidence for an imino intermediate in the T4 endonuclease V reaction

28 September 2017

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Random recombination of antibody single chain Fv sequences after fragmentation with DNasel in the presence of Mn2+

28 September 2017

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Simultaneous synthesis of human-, mouse- and chimeric epidermal growth factor genes via 'hybrid gene synthesis' approach

28 September 2017

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Formamidopyrimidine-DNA glycosylase of Escherichia coli: cloning and sequencing of the fpg structural gene and overproduction of the protein

28 September 2017

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Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases

28 September 2017

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DNA recombination during PCR.

28 September 2017

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Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity

28 September 2017

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Analysis of class II (hydrolytic) and class I (β-lyase) apurinic/apyrimidinic endonucleases with a synthetic DNA substrate

28 September 2017

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Randomization of genes by PCR mutagenesis

28 September 2017

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A new endonuclease from Escherichia coli acting at apurinic sites in DNA

28 September 2017

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Recursive PCR: a novel technique for total gene synthesis

28 September 2017

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Structural perturbation and compensation by directed evolution at physiological temperature leads to thermostabilization of beta-lactamase.

31 May 2018

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Helix-stabilized fv (hsfv) antibody fragments: substituting the constant domains of a fab fragment for a heterodimeric coiled-coil domain 1 1Edited by I. A. Wilson

28 November 2018

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Modeling DNA mutation and recombination for directed evolution experiments

28 November 2018

1 reference

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[Isolation and properties of DNA polymerase from extreme thermophylic bacteria Thermus aquaticus YT-1]

28 November 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/16061932

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1093/NAR/GNI116

1 reference

4 July 2017

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4 July 2017

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