The following is part of the review I'm writing for chloride channels. Not all of this will end up in the wiki.

Chloride channels are a group of ion channels that conduct anions. These channels act as pores through which chloride and other anions may pass through a lipid bilayer down their electrochemical gradient. Though many different anions can pass through these channels, chloride ions are the most abundant anion in their environment. Thus these channels have been named to reflect the primary ion associated with their use.^[1]

Several gene families contribute to the diversity of chloride channels. Variants described possess different structures, regulation, location, and activity.^[1]

The CLC family of chloride channels can be found in every branch of life. Members of the CLC gene family fall into two functional groups: chloride channels and H⁺/Cl^- exchangers. The channels are found in plasma membranes while the exchangers lie in membranes of intracellular organelles.^[2] Despite differential functions and locations, the structure is highly conserved between both groups.^[3]

The CLC channel structure has not yet been resolved by x-ray crystallography, however the structure of the CLC exchangers has been. Because the primary structure of the channels and exchangers are so similar, most assumptions about the structure of the channels are based on the structure established for the bacterial exchangers.^[3]

Each channel or exchanger is composed of two similar subunits—a dimer—each subunit containing one pore. The proteins are formed from two copies of the same protein—a homodimer—though scientists have artificially combined subunits from different channels to form heterodimers. Each subunit binds ions independently of the other, meaning conduction or exchange occur independently in each subunit.^[4]

Each subunit consists of two related halves oriented in opposite directions, forming an ‘antiparallel’ structure. These halves come together to form the anion pore.^[3] The pore has a filter through which chloride and other anions can pass, but lets little else through. These water-filled pores filter anions via three binding sites—S_int, S_cen, and S_ext—which bind chloride and other anions. The names of these binding sites correspond to their positions within the membrane. S_int is exposed to intracellular fluid, S_cen lies inside the membrane or in the center of the filter, and S_ext is exposed to extracellular fluid.^[4] Each binding site binds different chloride anions simultaneously. In the exchangers, these chloride ions do not interact strongly with one another, due to compensating interactions with the protein. In the channels, the protein does not shield chloride ions at one binding site from the neighboring negatively charged chlorides.^[2] Each negative charge exerts a repulsive force on the negative charges next to it. Researchers have suggested that this mutual repulsion contributes to the high rate of conduction through the pore.^[3]

CLC transporters shuttle H⁺ across the membrane. The H⁺ pathway in CLC transporters utilizes two glutamate residues—one on the extracellular side, Glu_ex, and one on the intracellular side, Glu_in. Glu_ex also serves to regulate chloride exchange between the protein and extracellular solution. This means that the chloride and the proton share a common pathway on the extracellular side, but diverge on the intracellular side.^[2]

CLC channels also have dependence on H⁺, but for gating rather than Cl^- exchange. Instead of utilizing gradients to exchange two Cl^- for one H⁺, the CLC channels transport one H⁺ while simultaneously transporting millions of anions.^[2]

Eukaryotic CLC channels also contain cytoplasmic domains. These domains have a pair of CBS motifs, whose function is not fully characterized yet.^[3]Though the precise function of these domains is not fully characterized, their importance is illustrated by the pathologies resulting from their mutation. Thomsen’s disease, Dent’s disease, infantile malignant osteopetrosis, and Bartter’s syndrome are all genetic disorders due to such mutations.^[2]

At least one role of the cytoplasmic CBS domains regards regulation via adenosine nucleotides. Particular CLC transporters and proteins have modulated activity when bound with ATP, ADP, AMP, or adenosine at the CBS domains. The specific effect is unique to each protein, but the implication is that certain CLC transporters and proteins are sensitive to the metabolic state of the cell.^[2]

The S_cen acts as the primary selectivity filter for most CLC proteins, allowing the following anions to pass through, from most selected to least selected: SCN^-, Cl^-, Br^-, NO₃^-, I^-. Altering a serine residue at the selectivity filter, labeled Ser_cen, to a different amino acid alters the selectivity.^[2]

Gating occurs through two mechanisms: protopore or fast gating and common or slow gating. Common gating involves both protein subunits closing their pores at the same time (cooperation), while protopore gating involves independent opening and closing of each pore^[3]. As the names imply, fast gating occur at a much faster rate than slow gating. Precise molecular mechanisms for gating are still being studied.

For the channels, when the slow gate is closed, no ions permeate through the pore. When the slow gate is open, the fast gates open spontaneously and independently of one another. Thus, the protein could have both gates open, or both gates closed, or just one of the two gates open. Single-channel patch-clamp studies demonstrated this biophysical property even before the dual-pore structure of CLC channels had been resolved. Each fast gate opens independently of the other and the ion conductance measured during these studies reflects a binomial distribution.^[4]

H⁺ transport promotes opening of the common gate in CLC channels. For every opening and closing of the common gate, one H⁺ is transported across the membrane. The common gate is also affected by the bonding of adenosine nucleotides to the intracellular CBS domains. Inhibition or activation of the protein by these domains is specific to each protein.^[2]

This is a user sandbox of Drummerdude987. A user sandbox is a subpage of the user's user page. It serves as a testing spot and page development space for the user and is not an encyclopedia article.

The following will be the new article, with my changes indicated:

Chloride channels are a superfamily of poorly understood ion channels for chloride. These channels may conduct many different ions, but are named for chloride because its concentration in vivo is much higher than other anions.^[1] Several families of voltage-gated channels and ligand-gatedchannels (e.g., the CaCC families) have been characterized in humans.

Voltage-gated chloride channels display a variety of important physiological and cellular roles that include regulation of pH, volume homeostasis, organic solute transport, cell migration, cell proliferation and differentiation. Based on sequence homology the chloride channels can be subdivided into a number of groups.

Voltage-gated chloride channels are important for setting cell resting membrane potential and maintaining proper cell volume. These channels conduct Cl⁻ as well as other anions such as HCO₃⁻, I⁻, SCN⁻, and NO₃⁻. The structure of these channels are not like other known channels. The chloride channel subunits contain between 1 and 12 transmembrane segments. Some chloride channels are activated only by voltage (i.e., voltage-gated), while others are activated by Ca²⁺, other extracellular ligands, or pH.^[5]

The CLC family of chloride channels contains 10 or 12 transmembrane helices. Each protein forms a single pore. It has been shown that some members of this family form homodimers. In terms of primary structure, they are unrelated to known cation channels or other types of anion channels. Three CLC subfamilies are found in animals. CLCN1is involved in setting and restoring the resting membrane potential of skeletal muscle, while other channels play important parts in solute concentration mechanisms in the kidney.^[4] These proteins contain two CBS domains. Chloride channels are also important for maintaining safe ion concentrations within plant cells.^[6]

The CLC channel structure has not yet been resolved by x-ray crystallography, however the structure of the CLC exchangers has been. Because the primary structure of the channels and exchangers are so similar, most assumptions about the structure of the channels are based on the structure established for the bacterial exchangers.^[3]

Each channel or exchanger is composed of two similar subunits—a dimer—each subunit containing one pore. The proteins are formed from two copies of the same protein—a homodimer—though scientists have artificially combined subunits from different channels to form heterodimers. Each subunit binds ions independently of the other, meaning conduction or exchange occur independently in each subunit.^[4]

Each subunit consists of two related halves oriented in opposite directions, forming an ‘antiparallel’ structure. These halves come together to form the anion pore.^[3] The pore has a filter through which chloride and other anions can pass, but lets little else through. These water-filled pores filter anions via three binding sites—S_int, S_cen, and S_ext—which bind chloride and other anions. The names of these binding sites correspond to their positions within the membrane. S_int is exposed to intracellular fluid, S_cen lies inside the membrane or in the center of the filter, and S_ext is exposed to extracellular fluid.^[4] Each binding site binds different chloride anions simultaneously. In the exchangers, these chloride ions do not interact strongly with one another, due to compensating interactions with the protein. In the channels, the protein does not shield chloride ions at one binding site from the neighboring negatively charged chlorides.^[2] Each negative charge exerts a repulsive force on the negative charges next to it. Researchers have suggested that this mutual repulsion contributes to the high rate of conduction through the pore.^[3]

CLC transporters shuttle H⁺ across the membrane. The H⁺ pathway in CLC transporters utilizes two glutamate residues—one on the extracellular side, Glu_ex, and one on the intracellular side, Glu_in. Glu_ex also serves to regulate chloride exchange between the protein and extracellular solution. This means that the chloride and the proton share a common pathway on the extracellular side, but diverge on the intracellular side.^[2]

CLC channels also have dependence on H⁺, but for gating rather than Cl^- exchange. Instead of utilizing gradients to exchange two Cl^- for one H⁺, the CLC channels transport one H⁺ while simultaneously transporting millions of anions.^[2] This corresponds with one cycle of the slow gate.

Eukaryotic CLC channels also contain cytoplasmic domains. These domains have a pair of CBS motifs, whose function is not fully characterized yet.^[3]Though the precise function of these domains is not fully characterized, their importance is illustrated by the pathologies resulting from their mutation. Thomsen’s disease, Dent’s disease, infantile malignant osteopetrosis, and Bartter’s syndrome are all genetic disorders due to such mutations.^[2]

At least one role of the cytoplasmic CBS domains regards regulation via adenosine nucleotides. Particular CLC transporters and proteins have modulated activity when bound with ATP, ADP, AMP, or adenosine at the CBS domains. The specific effect is unique to each protein, but the implication is that certain CLC transporters and proteins are sensitive to the metabolic state of the cell.^[2]

The S_cen acts as the primary selectivity filter for most CLC proteins, allowing the following anions to pass through, from most selected to least selected: SCN^-, Cl^-, Br^-, NO₃^-, I^-. Altering a serine residue at the selectivity filter, labeled Ser_cen, to a different amino acid alters the selectivity.^[2]

Gating occurs through two mechanisms: protopore or fast gating and common or slow gating. Common gating involves both protein subunits closing their pores at the same time (cooperation), while protopore gating involves independent opening and closing of each pore^[3]. As the names imply, fast gating occur at a much faster rate than slow gating. Precise molecular mechanisms for gating are still being studied.

For the channels, when the slow gate is closed, no ions permeate through the pore. When the slow gate is open, the fast gates open spontaneously and independently of one another. Thus, the protein could have both gates open, or both gates closed, or just one of the two gates open. Single-channel patch-clamp studies demonstrated this biophysical property even before the dual-pore structure of CLC channels had been resolved. Each fast gate opens independently of the other and the ion conductance measured during these studies reflects a binomial distribution.^[4]

H⁺ transport promotes opening of the common gate in CLC channels. For every opening and closing of the common gate, one H⁺ is transported across the membrane. The common gate is also affected by the bonding of adenosine nucleotides to the intracellular CBS domains. Inhibition or activation of the protein by these domains is specific to each protein.^[2]

The CLC channels allow chloride to flow down its electrochemical gradient, when open. These channels are expressed on the cell membrane. CLC channels contribute to the excitability of these membranes as well as transport ions across the membrane.^[4]

The CLC exchangers are localized to intracellular components like endosomes or lysosomes and help regulate the pH of their compartments.^[4]

Bartter's syndrome, which is associated with renal salt wasting and hypokalemic alkalosis, is due to the defective transport of chloride ions and associated ions in the thick ascending loop of Henle. CLCNKB has been implicated.^[7]

Another inherited disease that affects the kidney organs is Dent's Disease, characterised by low molecular weight proteinuria and hypercalciuria where mutations in CLCN5 are implicated.^[7]

Thomsen disease is associated with dominant mutations and Becker disease with recessive mutations in CLCN1.^[7]

CFTR is a chloride channel belonging to the superfamily of ABC transporters. Each channel has two transmembrane domains and two nucleotide binding domains. ATP binding to both nucleotide binding domains causes changes these domains to associate, further causing changes that open up the ion pore. When ATP is hydrolyzed, the nucleotide binding domains dissociate again and the pore closes.^[8]

Cystic fibrosis is caused by mutations in the CFTR gene, which prevents the proper folding of the protein and subsequent degradation, resulting in decreased numbers of chloride channels in the body. This causes the buildup of mucus in the body and chronic infections.^[8]

• CLIC family
• GABA_A
• Glycine Receptor
• Calcium-activated chloride channel

Some organic materials disrupt chloride channels in fleas, causing death. Selamectin is the active ingredient in Revolution, a topical insecticide and antihelminthic used on dogs and cats. Selamectin works by replacing glutamate which normally interacts with receptors that open chloride channels at muscle synapses found in parasites. Unlike glutamate, selamectin activates the chloride current without desensitization, thereby producing prolonged hyperpolarization and impaired muscle contraction.