DDX50

DDX50
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2E29
Identifikatori
Aliasi	DDX50
Vanjski ID-jevi	OMIM: 610373 MGI: 2182303 HomoloGene: 56986 GeneCards: DDX50
Lokacija gena (čovjek)
Hrom.	Hromosom 10 (čovjek)
Kraj	68,946,847 bp
Lokacija gena (miš)
Hrom.	Hromosom 10 (miš)
Kraj	62,486,997 bp
Obrazac RNK ekspresije
	; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• nucleotide binding; • hydrolase activity; • ATP binding; • GO:0008026 helicase activity; • nucleic acid binding; • vezivanje sa RNK;
Ćelijska komponenta	• ćelijska membrana; • membrana; • jedro; • Jedarce; • citoplazma;
Biološki proces	• RNA secondary structure unwinding;
	Izvori:Amigo / QuickGO
Ortolozi
	79009
	94213
	ENSG00000107625
	ENSMUSG00000020076
	Q9BQ39
	Q99MJ9
	NM_024045
	NM_053183; NM_001358819; NM_001358820
	NP_076950
	NP_444413; NP_001345748; NP_001345749
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

ATP-ovisna RNK-helikaza DDX50 je enzim koji je kod ljudi kodiran genom DDX50.^[5]^[6]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 737 aminokiselina, а molekulska težina 82 565^[7]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MPGKLLWGDI	MELEAPLEES	ESQKKERQKS	DRRKSRHHYD	SDEKSETREN
GVTDDLDAPK	AKKSKMKEKL	NGDTEEGFNR	LSDEFSKSHK	SRRKDLPNGD
IDEYEKKSKR	VSSLDTSTHK	SSDNKLEETL	TREQKEGAFS	NFPISEETIK
LLKGRGVTYL	FPIQVKTFGP	VYEGKDLIAQ	ARTGTGKTFS	FAIPLIERLQ
RNQETIKKSR	SPKVLVLAPT	RELANQVAKD	FKDITRKLSV	ACFYGGTSYQ
SQINHIRNGI	DILVGTPGRI	KDHLQSGRLD	LSKLRHVVLD	EVDQMLDLGF
AEQVEDIIHE	SYKTDSEDNP	QTLLFSATCP	QWVYKVAKKY	MKSRYEQVDL
VGKMTQKAAT	TVEHLAIQCH	WSQRPAVIGD	VLQVYSGSEG	RAIIFCETKK
NVTEMAMNPH	IKQNAQCLHG	DIAQSQREIT	LKGFREGSFK	VLVATNVAAR
GLDIPEVDLV	IQSSPPQDVE	SYIHRSGRTG	RAGRTGICIC	FYQPRERGQL
RYVEQKAGIT	FKRVGVPSTM	DLVKSKSMDA	IRSLASVSYA	AVDFFRPSAQ
RLIEEKGAVD	ALAAALAHIS	GASSFEPRSL	ITSDKGFVTM	TLESLEEIQD
VSCAWKELNR	KLSSNAVSQI	TRMCLLKGNM	GVCFDVPTTE	SERLQAEWHD
SDWILSVPAK	LPEIEEYYDG	NTSSNSRQRS	GWSSGRSGRS	GRSGGRSGGR
SGRQSRQGSR	SGSRQDGRRR	SGNRNRSRSG	GHKRSFD

Funkcija

Proteini DEAD-kutija, karakterizirani konzerviranim motivom Asp-Glu-Ala-Asp (DEAD), su pretpostavljene RNK helikaze. Uključeni su u brojne ćelijske procese koji obuhvataju promjenu sekundarnu strukturu RNK, kao što su inicijacija translacije, jedarno i mitohondrijsko spajanje i ribosoma i sklapanje splajsosoma. Na osnovu njihovih obrazaca distribucije, vjeruje se da su neki članovi ove porodice proteina iz DEAD-kutij uključeni u embriogenezu, spermatogenezu i ćelijski rast i diobu. Ovaj gen kodira DEAD-boksni enzim koji može biti uključen u sintezu ili preradu ribosomske RNK. Ovaj gen i DDX21, koji se također naziva RH-II/GuA, imaju slične genomske strukture i u tandemskoj su orijentaciji na hromosomu 10, što sugerira da su dva gena nastala duplikacijom gena u evoluciji. Ovaj gen ima pseudogene na hromosomima 2, 3 i 4. Alternativna prerada ovog gena stvara višestruke varijante transkripta, ali priroda pune dužine svih ostalih varijanti ni nije definirana.^[6]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000107625 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000020076 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Valdez BC, Yang H, Hong E, Sequitin AM (Mar 2002). "Genomic structure of newly identified paralogue of RNA helicase II/Gu: detection of pseudogenes and multiple alternatively spliced mRNAs". Gene. 284 (1–2): 53–61. doi:10.1016/S0378-1119(01)00888-5. PMID 11891046.
^ ^a ^b "Entrez Gene: DDX50 DEAD (Asp-Glu-Ala-Asp) box polypeptide 50".
^ "UniProt, Q9BQ39". Pristupljeno 31. 8. 2021.

Dopunska literatura

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. S2CID 14132033.
Scherl A, Couté Y, Déon C, et al. (2003). "Functional proteomic analysis of human nucleolus". Mol. Biol. Cell. 13 (11): 4100–9. doi:10.1091/mbc.E02-05-0271. PMC 133617. PMID 12429849.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.

Vanjski linkovi

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000107625 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000020076 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid11891046-5] Valdez BC, Yang H, Hong E, Sequitin AM (Mar 2002). "Genomic structure of newly identified paralogue of RNA helicase II/Gu: detection of pseudogenes and multiple alternatively spliced mRNAs". Gene. 284 (1–2): 53–61. doi:10.1016/S0378-1119(01)00888-5. PMID 11891046.

[entrez-6] "Entrez Gene: DDX50 DEAD (Asp-Glu-Ala-Asp) box polypeptide 50".

[UniProt-7] "UniProt, Q9BQ39". Pristupljeno 31. 8. 2021.

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p r u Enzimi
Teme	Aktivno mjesto Alosterna regulacija Difuzijski limitirani enzim EC broj Enzimska kataliza Inhibicija enzimskih reakcija Kinetika enzima Koenzim Kooperativnost Lineweaver–Burkov dijagram Michaelis–Mentenova kinetika Mjesto vezanja Spisak enzima
Tipovi	EC1 Oksidoreduktaze/spisak EC2 Transferaze/spisak EC3 Hidrolaze/spisak EC4 Lijaze/spisak EC5 Izomeraze/spisak EC6 Ligaze/spisak