Gene disruption. (Q52546440)

3 May 2018

title

Gene disruption. (English)

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3 May 2018

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3 May 2018

author name string

Linda Riles

series ordinal

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3 May 2018

Johannes H Hegemann

series ordinal

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3 May 2018

language of work or name

English

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publication date

1 January 2002

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3 May 2018

published in

Methods in Enzymology

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3 May 2018

volume

350

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3 May 2018

page(s)

290-315

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3 May 2018

Transformation of Saccharomyces cerevisiae with nonhomologous DNA: illegitimate integration of transforming DNA into yeast chromosomes and in vivo ligation of transforming DNA to mitochondrial DNA sequences

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A method for performing precise alterations in the yeast genome using a recyclable selectable marker

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Gene disruption with PCR products in Saccharomyces cerevisiae

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Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis

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PCR-mediated direct gene disruption in Schizosaccharomyces pombe

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A recyclable Candida albicans URA3 cassette for PCR product-directed gene disruptions.

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PCR- and ligation-mediated synthesis of split-marker cassettes with long flanking homology regions for gene disruption in Candida albicans.

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PCR-based gene targeting in the filamentous fungus Ashbya gossypii.

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Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast

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Micro-homology mediated PCR targeting inSaccharomyces cerevisiae

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PCR- and ligation-mediated synthesis of marker cassettes with long flanking homology regions for gene disruption in Saccharomyces cerevisiae

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A new efficient gene disruption cassette for repeated use in budding yeast

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Heterologous URA3MX cassettes for gene replacement in Saccharomyces cerevisiae.

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Sticky-end polymerase chain reaction method for systematic gene disruption in Saccharomyces cerevisiae

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5-Fluoroorotic acid as a selective agent in yeast molecular genetics

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A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

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Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast

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A novel strategy for constructing N-terminal chromosomal fusions to green fluorescent protein in the yeast Saccharomyces cerevisiae.

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Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions.

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7 January 2021

10.1016/S0076-6879(02)50970-8

Identifiers

DOI

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3 May 2018

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3 May 2018