Cloning-free PCR-based allele replacement methods (Q35020750)

5 August 2017

title

Cloning-free PCR-based allele replacement methods (English)

1 reference

5 August 2017

R Rothstein

0 references

N Erdeniz

1 reference

5 August 2017

U H Mortensen

series ordinal

1 reference

5 August 2017

publication date

1 December 1997

1 reference

5 August 2017

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5 August 2017

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5 August 2017

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5 August 2017

page(s)

1174-1183

1 reference

The complete set of predicted genes from Saccharomyces cerevisiae in a readily usable form

5 August 2017

cites work

1 reference

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Removal of polymerase-produced mutant sequences from PCR products

8 July 2018

1 reference

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Use of polymerase chain reaction epitope tagging for protein tagging in Saccharomyces cerevisiae

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

A rapid and reliable DNA preparation method for screening a large number of yeast clones by polymerase chain reaction

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

Yeast kar1 mutants provide an effective method for YAC transfer to new hosts

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

A mutation in the gene encoding the Saccharomyces cerevisiae single-stranded DNA-binding protein Rfa1 stimulates a RAD52-independent pathway for direct-repeat recombination

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

A method for performing precise alterations in the yeast genome using a recyclable selectable marker

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

Lethal disruption of the yeast actin gene by integrative DNA transformation.

8 July 2018

1 reference

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[12] One-step gene disruption in yeast

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

Sequence of the Kluyveromyces lactis URA3 gene

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

5-Fluoroorotic acid as a selective agent in yeast molecular genetics

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

The genetic control of direct-repeat recombination in Saccharomyces: the effect of rad52 and rad1 on mitotic recombination at GAL10, a transcriptionally regulated gene

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae

8 July 2018

1 reference

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A defect in mismatch repair in Saccharomyces cerevisiae stimulates ectopic recombination between homeologous genes by an excision repair dependent process

8 July 2018

1 reference

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Rapid assessment of S. cerevisiae mating type by PCR.

8 July 2018

1 reference

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The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

A mutant of Saccharomyces cerevisiae defective for nuclear fusion

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

Replacement of chromosome segments with altered DNA sequences constructed in vitro

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

Transformation of Saccharomyces cerevisiae with nonhomologous DNA: illegitimate integration of transforming DNA into yeast chromosomes and in vivo ligation of transforming DNA to mitochondrial DNA sequences

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

Plasmid construction by homologous recombination in yeast.

25 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=310678

Human homologues of yeast helicase

25 October 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/9414323

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Genetic applications of yeast transformation with linear and gapped plasmids

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/9414323

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

One-step gene disruption in yeast

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/9414323

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1101/GR.7.12.1174

1 reference

5 August 2017

1 reference

5 August 2017

1 reference