Site-specific incorporation of fluorotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation. (Q50989020)

26 March 2018

title

Site-specific incorporation of fluorotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation. (English)

1 reference

26 March 2018

0 references

Joanne Stubbe

1 reference

26 March 2018

Mohammad Seyedsayamdost

series ordinal

object named as

Mohammad R Seyedsayamdost

1 reference

26 March 2018

author name string

Cyril S Yee

series ordinal

1 reference

26 March 2018

publication date

1 January 2007

1 reference

26 March 2018

published in

Nature Protocols

1 reference

26 March 2018

volume

1 reference

26 March 2018

issue

1 reference

26 March 2018

page(s)

1225-1235

1 reference

26 March 2018

Protein Radicals in Enzyme Catalysis

cites work

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Ribonucleotide reductases

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Ribonucleotide reductases

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Harnessing free radicals: formation and function of the tyrosyl radical in ribonucleotide reductase

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Radical initiation in the class I ribonucleotide reductase: long-range proton-coupled electron transfer?

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Structure of ribonucleotide reductase protein R1

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Three-dimensional structure of the free radical protein of ribonucleotide reductase

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Displacement of the tyrosyl radical cofactor in ribonucleotide reductase obtained by single-crystal high-field EPR and 1.4-A x-ray data

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Two conserved tyrosine residues in protein R1 participate in an intermolecular electron transfer in ribonucleotide reductase.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Preserved catalytic activity in an engineered ribonucleotide reductase R2 protein with a nonphysiological radical transfer pathway. The importance of hydrogen bond connections between the participating residues

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Evidence by site-directed mutagenesis supports long-range electron transfer in mouse ribonucleotide reductase.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Evidence by mutagenesis that Tyr(370) of the mouse ribonucleotide reductase R2 protein is the connecting link in the intersubunit radical transfer pathway

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

In vivo assay for low-activity mutant forms of Escherichia coli ribonucleotide reductase

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Mono-, di-, tri-, and tetra-substituted fluorotyrosines: new probes for enzymes that use tyrosyl radicals in catalysis

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

The function and characteristics of tyrosyl radical cofactors

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Generation of the R2 subunit of ribonucleotide reductase by intein chemistry: insertion of 3-nitrotyrosine at residue 356 as a probe of the radical initiation process

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

pH Rate profiles of FnY356-R2s (n = 2, 3, 4) in Escherichia coli ribonucleotide reductase: evidence that Y356 is a redox-active amino acid along the radical propagation pathway

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Protein splicing and related forms of protein autoprocessing

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Protein splicing: self-splicing of genetically mobile elements at the protein level.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Protein ligation: an enabling technology for the biophysical analysis of proteins

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Protein splicing mechanisms and applications

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Protein splicing and autoproteolysis mechanisms.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Expanding the Genetic Code

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

An expanding genetic code

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Semisynthesis of proteins by expressed protein ligation

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Recent advances in the application of expressed protein ligation to protein engineering

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Semisynthetic proteins in mechanistic studies: using chemistry to go where nature can't

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Occurrence and Minimization of Cysteine Racemization during Stepwise Solid-Phase Peptide Synthesis1,2

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.159

31 March 2020

Identifiers

DOI

10.1038/NPROT.2007.159

1 reference

26 March 2018

1 reference

26 March 2018