Protein ligation: an enabling technology for the biophysical analysis of proteins (Q34529929)

2 August 2017

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title

Protein ligation: an enabling technology for the biophysical analysis of proteins (English)

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2 August 2017

Vasant Muralidharan

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2 August 2017

Tom W Muir

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2 August 2017

publication date

1 June 2006

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2 August 2017

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2 August 2017

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2 August 2017

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2 August 2017

page(s)

429-438

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Manipulating proteins with chemistry: a cross-section of chemical biology

2 August 2017

cites work

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7 January 2021

Synthesis of proteins by native chemical ligation

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7 January 2021

Semisynthesis of proteins by expressed protein ligation

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7 January 2021

Protein splicing and related forms of protein autoprocessing

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7 January 2021

NMR observation of selected segments in a larger protein: central-segment isotope labeling through intein-mediated ligation

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7 January 2021

Protein semisynthesis and expressed protein ligation: chasing a protein's tail

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7 January 2021

Semisynthesis of cytotoxic proteins using a modified protein splicing element

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7 January 2021

Intein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicing

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7 January 2021

Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies

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7 January 2021

The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins

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7 January 2021

Circular β-lactamase: stability enhancement by cyclizing the backbone

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7 January 2021

Production of cyclic peptides and proteins in vivo

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7 January 2021

Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803

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7 January 2021

Peptide chemical ligation inside living cells: in vivo generation of a circular protein domain.

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7 January 2021

Rescuing a destabilized protein fold through backbone cyclization

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7 January 2021

Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus.

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7 January 2021

Structural requirements for the biosynthesis of backbone cyclic peptide libraries.

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7 January 2021

Retrovirally delivered random cyclic Peptide libraries yield inhibitors of interleukin-4 signaling in human B cells

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7 January 2021

Stabilization of native protein fold by intein-mediated covalent cyclization

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7 January 2021

Chemical synthesis of peptides and proteins

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7 January 2021

Covalent capture: a new tool for the purification of synthetic and recombinant polypeptides

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7 January 2021

A one-pot total synthesis of crambin

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7 January 2021

Oligopeptide biases in protein sequences and their use in predicting protein coding regions in nucleotide sequences

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7 January 2021

Semisynthesis and folding of the potassium channel KcsA

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7 January 2021

Synthesis of peptides and proteins without cysteine residues by native chemical ligation combined with desulfurization

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7 January 2021

Extending synthetic access to proteins with a removable acyl transfer auxiliary.

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7 January 2021

A "traceless" Staudinger ligation for the chemoselective synthesis of amide bonds

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7 January 2021

Staudinger ligation: a peptide from a thioester and azide.

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7 January 2021

Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein

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7 January 2021

Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein

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7 January 2021

Fusions to self-splicing inteins for protein purification

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7 January 2021

Protein synthesis by native chemical ligation: expanded scope by using straightforward methodology

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7 January 2021

Native chemical ligation through in situ O to S acyl shift

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7 January 2021

Cysteine-derived s-protected oxazolidinones: potential chemical devices for the preparation of peptide thioesters

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7 January 2021

Expressed protein ligation: a general method for protein engineering

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7 January 2021

Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR

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7 January 2021

The leucine zipper domain controls the orientation of AP-1 in the NFAT.AP-1.DNA complex

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7 January 2021

New methods for proteomic research: preparation of proteins with N-terminal cysteines for labeling and conjugation

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7 January 2021

Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution

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7 January 2021

Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation

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7 January 2021

A genetic system yields self-cleaving inteins for bioseparations

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7 January 2021

Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction

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7 January 2021

Domain-specific incorporation of noninvasive optical probes into recombinant proteins

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7 January 2021

Direct production of proteins with N-terminal cysteine for site-specific conjugation

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7 January 2021

Development of a tandem protein trans-splicing system based on native and engineered split inteins

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7 January 2021

Control of protein splicing by intein fragment reassembly.

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7 January 2021

Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein

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7 January 2021

Synthetic two-piece and three-piece split inteins for protein trans-splicing

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7 January 2021

Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803

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7 January 2021

Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies.

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7 January 2021

Protein splicing triggered by a small molecule

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7 January 2021

Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing

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7 January 2021

Protein semi-synthesis in living cells.

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7 January 2021

Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes

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7 January 2021

Intein-mediated assembly of a functional beta-glucuronidase in transgenic plants

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7 January 2021

Pi release from eIF2, not GTP hydrolysis, is the step controlled by start-site selection during eukaryotic translation initiation

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7 January 2021

A conformational change in the eukaryotic translation preinitiation complex and release of eIF1 signal recognition of the start codon

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7 January 2021

Motion of carboxyl terminus of Galpha is restricted upon G protein activation. A solution NMR study using semisynthetic Galpha subunits.

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7 January 2021

Conformational change of H+-ATPase beta monomer revealed on segmental isotope labeling NMR spectroscopy

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7 January 2021

Positioning membrane proteins by novel protein engineering and biophysical approaches

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7 January 2021

Crystal Structure of a Phosphorylated Smad2

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7 January 2021

Smad3 allostery links TGF-beta receptor kinase activation to transcriptional control

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7 January 2021

Structural basis of heteromeric smad protein assembly in TGF-beta signaling

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7 January 2021

Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase

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7 January 2021

Total chemical synthesis and electrophysiological characterization of mechanosensitive channels from Escherichia coli and Mycobacterium tuberculosis

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7 January 2021

Glycine as a D-amino acid surrogate in the K(+)-selectivity filter

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7 January 2021

The Structure of the Potassium Channel: Molecular Basis of K+ Conduction and Selectivity

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7 January 2021

Using protein-DNA chimeras to detect and count small numbers of molecules

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7 January 2021

Synthesis of protein-nucleic acid conjugates by expressed protein ligation

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7 January 2021

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

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7 January 2021

Molecular dissection of the Mycobacterium tuberculosis RecA intein: design of a minimal intein and of a trans-splicing system involving two intein fragments

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7 January 2021

The in vitro ligation of bacterially expressed proteins using an intein from Methanobacterium thermoautotrophicum

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7 January 2021

Chemical ligation of unprotected peptides directly from a solid support

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7 January 2021

Solid Phase Synthesis of Peptide C-Terminal Thioesters by Fmoc/t-Bu Chemistry

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7 January 2021

Backbone Amide Linker (BAL) Strategy forNα-9-Fluorenylmethoxycarbonyl (Fmoc) Solid-Phase Synthesis of Unprotected Peptidep-Nitroanilides and Thioesters1

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7 January 2021

Facile, fmoc-compatible solid-phase synthesis of peptide C-terminal thioesters

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7 January 2021

Fmoc-based synthesis of peptide alpha-thioesters using an aryl hydrazine support

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7 January 2021

InBase: the Intein Database

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7 January 2021

Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step

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7 January 2021

Simple bioseparations using self-cleaving elastin-like polypeptide tags

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7 January 2021

Self-cleavable stimulus responsive tags for protein purification without chromatography

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7 January 2021

Split dnaE genes encoding multiple novel inteins in Trichodesmium erythraeum

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7 January 2021

Mutational analysis of protein splicing, cleavage, and self-association reactions mediated by the naturally split Ssp DnaE intein

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7 January 2021

Constructing proteins by dovetailing unprotected synthetic peptides: backbone-engineered HIV protease

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7 January 2021

Construction of protein analogues by site-specific condensation of unprotected fragments

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7 January 2021

Chemoselective approaches to glycoprotein assembly

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7 January 2021

The growing impact of click chemistry on drug discovery

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7 January 2021

Native chemical ligation of hydrophobic [corrected] peptides in lipid bilayer systems

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7 January 2021

Total chemical synthesis of the integral membrane protein influenza A virus M2: role of its C-terminal domain in tetramer assembly.

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7 January 2021

Cell-permeable small molecule probes for site-specific labeling of proteins

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7 January 2021

Identifiers

DOI

10.1038/NMETH886

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Dimensions Publication ID

2 August 2017

1031061213

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