The Cryo-EM structure of a complete 30S translation initiation complex from Escherichia coli (Q33959135)

30 July 2017

title

The Cryo-EM structure of a complete 30S translation initiation complex from Escherichia coli (English)

1 reference

cryogenic electron microscopy

30 July 2017

main subject

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1 reference

Xabier Agirrezabala

1 reference

30 July 2017

Gorka Lasso

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30 July 2017

Mikel Valle

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30 July 2017

Pohl Milon

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David Gil

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Marina V Rodnina

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Patricia Julián

1 reference

30 July 2017

language of work or name

English

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publication date

5 July 2011

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30 July 2017

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30 July 2017

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30 July 2017

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30 July 2017

page(s)

e1001095

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Creative Commons Attribution 4.0 International

30 July 2017

start time

5 July 2011

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April 2022 Public Data File from Crossref

copyrighted

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cites work

The ribosome-bound initiation factor 2 recruits initiator tRNA to the 30S initiation complex

1 reference

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Autoregulatory systems controlling translation factor expression: thermostat-like control of translational accuracy

5 July 2018

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The Crystal Structure of the Ribosome Bound to EF-Tu and Aminoacyl-tRNA

5 July 2018

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GTP hydrolysis by IF2 guides progression of the ribosome into elongation

5 July 2018

1 reference

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Structure of ratcheted ribosomes with tRNAs in hybrid states

5 July 2018

1 reference

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Visualization of the hybrid state of tRNA binding promoted by spontaneous ratcheting of the ribosome

5 July 2018

1 reference

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Structure of the 30S translation initiation complex.

5 July 2018

1 reference

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Crystal structure of the intact archaeal translation initiation factor 2 demonstrates very high conformational flexibility in the alpha- and beta-subunits

5 July 2018

1 reference

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Spontaneous intersubunit rotation in single ribosomes.

5 July 2018

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Image processing for electron microscopy single-particle analysis using XMIPP.

5 July 2018

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Protein structure fitting and refinement guided by cryo-EM density

5 July 2018

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Interactions and dynamics of the Shine Dalgarno helix in the 70S ribosome

5 July 2018

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Methods for identifying compounds that specifically target translation

5 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors

5 July 2018

1 reference

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SPIRE: the SPIDER reconstruction engine

5 July 2018

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Structural basis for messenger RNA movement on the ribosome

5 July 2018

1 reference

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Structure of the 70S ribosome complexed with mRNA and tRNA

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Translation initiation factor IF2 interacts with the 30 S ribosomal subunit via two separate binding sites

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

How initiation factors tune the rate of initiation of protein synthesis in bacteria

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

The fidelity of translation initiation: reciprocal activities of eIF1, IF3 and YciH.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Mechanism for the disassembly of the posttermination complex inferred from cryo-EM studies

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

The cryo-EM structure of a translation initiation complex from Escherichia coli

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Initiation of protein synthesis in bacteria

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

UCSF Chimera--a visualization system for exploratory research and analysis

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Visualization of release factor 3 on the ribosome during termination of protein synthesis

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Position of eukaryotic initiation factor eIF1 on the 40S ribosomal subunit determined by directed hydroxyl radical probing

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Locking and unlocking of ribosomal motions

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

A conserved structural motif at the N terminus of bacterial translation initiation factor IF2

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

The path of messenger RNA through the ribosome

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Crystal structure of the ribosome at 5.5 A resolution

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Crystal structure of an initiation factor bound to the 30S ribosomal subunit

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Mapping the fMet-tRNA(f)(Met) binding site of initiation factor IF2.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

T-Coffee: A novel method for fast and accurate multiple sequence alignment

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

A ratchet-like inter-subunit reorganization of the ribosome during translocation

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Late events of translation initiation in bacteria: a kinetic analysis

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Structure of the fMet-tRNAfMet-binding domain of B.stearothermophilus initiation factor IF2

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Solution structure of the E. coli 70S ribosome at 11.5 A resolution

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Large-scale movement of elongation factor G and extensive conformational change of the ribosome during translocation

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Location of translational initiation factor IF3 on the small ribosomal subunit

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Crystal structure of methionyl-tRNAfMet transformylase complexed with the initiator formyl-methionyl-tRNAfMet.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

SWISS‐MODEL and the Swiss‐Pdb Viewer: An environment for comparative protein modeling

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Prokaryotic translation: the interactive pathway leading to initiation

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

X-ray crystallography shows that translational initiation factor IF3 consists of two compact alpha/beta domains linked by an alpha-helix

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Determination of the optimal aligned spacing between the Shine-Dalgarno sequence and the translation initiation codon of Escherichia coli mRNAs

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Selection of the initiator tRNA by Escherichia coli initiation factors.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Mutation at position 791 in Escherichia coli 16S ribosomal RNA affects processes involved in the initiation of protein synthesis

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Initiation of mRNA translation in prokaryotes

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Domains of initiator tRNA and initiation codon crucial for initiator tRNA selection by Escherichia coli IF3

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Translation initiation in Escherichia coli: sequences within the ribosome-binding site

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Light-scattering studies showing the effect of initiation factors on the reversible dissociation of Escherichia coli ribosomes

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Initial rate kinetic analysis of the mechanism of initiation complex formation and the role of initiation factor IF-3

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

Unfolding of mRNA secondary structure by the bacterial translation initiation complex

5 July 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PBIO.1001095

Conformational transition of initiation factor 2 from the GTP- to GDP-bound state visualized on the ribosome.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PBIO.1001095

The real-time path of translation factor IF3 onto and off the ribosome.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PBIO.1001095

Interaction of translation initiation factor 3 with the 30S ribosomal subunit

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PBIO.1001095

Disentangling conformational states of macromolecules in 3D-EM through likelihood optimization.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PBIO.1001095

Solution structure of the ribosome-binding domain of E. coli translation initiation factor IF3. Homology with the U1A protein of the eukaryotic spliceosome.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PBIO.1001095

Observation of intersubunit movement of the ribosome in solution using FRET.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PBIO.1001095

Translation initiation factor IF1 of Bacillus stearothermophilus and Thermus thermophilus substitute for Escherichia coli IF1 in vivo and in vitro without a direct IF1-IF2 interaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PBIO.1001095

How initiation factors maximize the accuracy of tRNA selection in initiation of bacterial protein synthesis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PBIO.1001095

Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PBIO.1001095

From stand-by to decoding site. Adjustment of the mRNA on the 30S ribosomal subunit under the influence of the initiation factors.

21 January 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3130014

A bacterial dynamin-like protein

29 October 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PBIO.1001095

E. coli translation initiation factor IF2 – an extremely conserved protein. Comparative sequence analysis of the infB gene in clinical isolates of E. coli

21 January 2018

1 reference

12 December 2020

Kinetic Checkpoint at a Late Step in Translation Initiation

1 reference

12 December 2020

Binding of Escherichia coli initiation factor IF2 to 30S ribosomal subunits: a functional role for the N-terminus of the factor

1 reference

12 December 2020

Characterization of the domains of E. coli initiation factor IF2 responsible for recognition of the ribosome

1 reference

12 December 2020

Effect of Escherichia coli initiation factors on the kinetics of N-AcPhe-tRNAPhe binding to 30S ribosomal subunits. A fluorescence stopped-flow study

1 reference

12 December 2020

10.1371/JOURNAL.PBIO.1001095

Identifiers

DOI

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30 July 2017

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30 July 2017

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