Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization (Q38621515)

4 September 2017

title

Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization (English)

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4 September 2017

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Alan R Horwitz

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4 September 2017

Brock Roberts

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4 September 2017

Amanda Haupt

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4 September 2017

Andrew Tucker

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4 September 2017

Tanya Grancharova

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4 September 2017

Joy Arakaki

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4 September 2017

Margaret A Fuqua

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4 September 2017

Angelique Nelson

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4 September 2017

Caroline Hookway

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4 September 2017

Susan A Ludmann

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4 September 2017

Irina A Mueller

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4 September 2017

Ruian Yang

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4 September 2017

Susanne M Rafelski

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4 September 2017

Ruwanthi N Gunawardane

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4 September 2017

publication date

16 August 2017

1 reference

Molecular Biology of the Cell

4 September 2017

published in

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describes a project that uses

4 September 2017

1 reference

Mechanistic basis of desmosome-targeted diseases

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https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

cGMP-Manufactured Human Induced Pluripotent Stem Cells Are Available for Pre-clinical and Clinical Applications

21 January 2018

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https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Derivation of pluripotent epiblast stem cells from mammalian embryos

21 January 2018

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https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Reconsidering pluripotency tests: do we still need teratoma assays?

21 January 2018

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https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

CellProfiler: image analysis software for identifying and quantifying cell phenotypes

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Human amniotic epithelial cell feeder layers maintain iPS cell pluripotency by inhibiting endogenous DNA methyltransferase 1.

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Repair of double-strand breaks by end joining

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Super-resolution imaging of fluorescently labeled, endogenous RNA Polymerase II in living cells with CRISPR/Cas9-mediated gene editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Multiplex genome engineering using CRISPR/Cas systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Tagging endogenous loci for live-cell fluorescence imaging and molecule counting using ZFNs, TALENs, and Cas9.

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Advances in fluorescence labeling strategies for dynamic cellular imaging

21 January 2018

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https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

STAR: ultrafast universal RNA-seq aligner

21 January 2018

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https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Rapid and efficient clathrin-mediated endocytosis revealed in genome-edited mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

The dynamics of postmitotic reassembly of the nucleolus

21 January 2018

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https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Gene Conversion Tracts from Double-Strand Break Repair in Mammalian Cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells

21 January 2018

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https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo

21 January 2018

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https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Functional organization of the yeast proteome by systematic analysis of protein complexes

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

The transience of transient overexpression

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Resources for the design of CRISPR gene editing experiments

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Actin and dynamin2 dynamics and interplay during clathrin-mediated endocytosis

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

gamma-Tubulin complexes and their role in microtubule nucleation

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Induced Pluripotent Stem Cells Meet Genome Editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Whole cell maps chart a course for 21st-century cell biology

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Global analysis of protein localization in budding yeast

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Repair of strand breaks by homologous recombination

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Versatile protein tagging in cells with split fluorescent protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Microtubule nucleation by γ-tubulin complexes

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

A scalable strategy for high-throughput GFP tagging of endogenous human proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Chemically defined, albumin-free human cardiomyocyte generation

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

RNA-guided human genome engineering via Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Cutadapt removes adapter sequences from high-throughput sequencing reads

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Isolation of single-base genome-edited human iPS cells without antibiotic selection

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Microtubule organization and function in epithelial cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives.

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Inhibition of β-catenin signaling respecifies anterior-like endothelium into beating human cardiomyocytes

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

A ZO1-GFP fusion protein to study the dynamics of tight junctions in living cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Introduction of double-strand breaks into the genome of mouse cells by expression of a rare-cutting endonuclease

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Inherited desmosomal disorders

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

The reticulon and DP1/Yop1p proteins form immobile oligomers in the tubular endoplasmic reticulum

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

T-box genes in early embryogenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Embryo-derived stem cells: of mice and men.

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

International coordination of large-scale human induced pluripotent stem cell initiatives: Wellcome Trust and ISSCR workshops white paper

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Aurora B-mediated abscission checkpoint protects against tetraploidization

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

New cell lines from mouse epiblast share defining features with human embryonic stem cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Embryonic stem cell lines derived from human blastocysts

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Organization of Non-centrosomal Microtubules in Epithelial Cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

SOX17 links gut endoderm morphogenesis and germ layer segregation

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Conditional expression of a truncated fragment of nonmuscle myosin II-A alters cell shape but not cytokinesis in HeLa cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Quality control: Genome maintenance in pluripotent stem cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Targeted genome editing across species using ZFNs and TALENs

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

The presenilin-1 ΔE9 mutation results in reduced γ-secretase activity, but not total loss of PS1 function, in isogenic human stem cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Alzheimer's disease in a dish: promises and challenges of human stem cell models

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

Pax6 is a human neuroectoderm cell fate determinant

21 January 2018

1 reference

https://api.crossref.org/works/10.1091%2FMBC.E17-03-0209

A robust method to derive functional neural crest cells from human pluripotent stem cells

21 January 2018

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PubMed Central

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5638588

Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration

4 November 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/28814507

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1091/MBC.E17-03-0209

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4 September 2017

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