Urat oksidaza
Urat oksidaza (EC 1.7.3.3, urinsko kiselinska oksidaza, urikaza, urikaza II, uratna-oksidaza) je enzim sa sistematskim imenom urat:kiseonik oksidoreduktaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Urat oksidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Urat oksidaza tetramer, Aspergillus flavus | |||||||||
| Identifikatori | |||||||||
| EC broj | 1.7.3.3 | ||||||||
| CAS broj | 2594291 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- urat + O2 +H2O 5-hidroksiizourat +H2O2
Enzimu iz zrna soje, plesni Aspergillus flavus i Bacillus subtilis ne sadrže bakar, niti druge jone prelaznih metala.
Reference
uredi- ^ London, M. & Hudson, P.B. (1956). „Purification and properties of solubilized uricase”. Biochim. Biophys. Acta. 21: 290—298. PMID 13363909.
- ^ Mahler, H.R., Hübscher, G. and Baum, H. (1955). „Studies on uricase. I. Preparation, purification, and properties of a cuproprotein”. J. Biol. Chem. 216: 625—641. PMID 13271340.
- ^ Robbins, K.C., Barnett, E.L. and Grant, N.H. (1955). „Partial purification of porcine liver uricase”. J. Biol. Chem. 216: 27—35. PMID 13252004.
- ^ Kahn, K. & Tipton, P.A. (1998). „Spectroscopic characterization of intermediates in the urate oxidase reaction”. Biochemistry. 37: 11651—11659. PMID 9709003.
- ^ Colloc'h, N., el Hajji, M., Bachet, B., L'Hermite, G., Schiltz, M., Prange, T., Castro, B. and Mornon, J.-P. (1997). „Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05 Å resolution”. Nat. Struct. Biol. 4: 947—952. PMID 9360612.
- ^ Imhoff, R.D., Power, N.P., Borrok, M.J. and Tipton, P.A. (2003). „General base catalysis in the urate oxidase reaction: evidence for a novel Thr-Lys catalytic diad”. Biochemistry. 42: 4094—4100. PMID 12680763.
Literatura
uredi- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze
uredi- Urate+oxidase na US National Library of Medicine Medical Subject Headings (MeSH)