[go: up one dir, main page]

Induction of apoptosis and necrosis in A549 cells by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP

Mutat Res. 2004 Sep 12;563(1):61-70. doi: 10.1016/j.mrgentox.2004.05.018.

Abstract

Non-small cell lung cancer (NSCLC) includes a group of tumors that respond poorly to drugs. cis-Diamminedichloroplatinum(II) (cis-DDP) toxicity still remains problematic, and not completely solved by the improvement of supportive care. Therefore, the cis-Pt(II) complex of 3-aminoflavone was selected from cis-DDP analogues with a more favourable toxic profile towards normal cells and at least similar or better anti-tumor activity in comparison with cis-DDP. The aim of this research is to compare the ability of the cis-Pt(II) complex of 3-aminoflavone and cis-DDP to induce apoptosis and necrosis in the human non-small cancer cell line A549. Trypan blue dye exclusion, fluorochrome staining (acridine orange/ethidium bromide double staining), MTT and TUNEL (TdT-mediated dUTP Nick-End Labeling) assays were used. The results obtained show that the cis-Pt(II) complex of 3-aminoflavone is more active in inducing apoptosis and necrosis and in decreasing viability in A549 cells than cis-DDP, which suggests that it could be a potential chemotherapeutic drug.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acridine Orange / metabolism
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / toxicity*
  • Apoptosis*
  • Cell Division
  • Cell Line, Tumor
  • Cell Survival
  • Cisplatin / chemistry
  • Cisplatin / metabolism
  • Cisplatin / toxicity*
  • Ethidium / metabolism
  • Flavonoids / chemistry
  • Flavonoids / metabolism*
  • Fluorescent Dyes / metabolism
  • Humans
  • In Situ Nick-End Labeling
  • Molecular Structure
  • Necrosis*
  • Tetrazolium Salts / metabolism
  • Thiazoles / metabolism

Substances

  • 3-aminoflavone
  • Antineoplastic Agents
  • Flavonoids
  • Fluorescent Dyes
  • Tetrazolium Salts
  • Thiazoles
  • Ethidium
  • thiazolyl blue
  • Acridine Orange
  • Cisplatin