A compared study on the purification of fetal and adult rat hepatocyte suspensions by biomagnetic isolation techniques and flow cytometry analysis

Cell Biol Int. 2001;25(12):1285-9. doi: 10.1006/cbir.2001.0814.

Authors

E Arza¹, F J Cubero, M S García-Barrutia, A Ortiz, P Maganto, N Mula, R M Arahuetes

Affiliation

¹ Departamento de Biología Animal II, Universidad Complutense, 28040 Madrid, Spain.

PMID: 11748922
DOI: 10.1006/cbir.2001.0814

Abstract

An analysis of liver cell populations from both adult and 21 day pregnancy rat fetuses (E21) was carried out. The results show that E21 hepatocytes express OX-43, as do endothelial cells but not adult hepatocytes. OX-43 could be used in future as a cell marker for the hepatocyte maturation.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal
Cells, Cultured
Female
Flow Cytometry
Hepatocytes / cytology*
Immunomagnetic Separation
Liver / cytology*
Liver / embryology
Male
Microscopy, Confocal
Pregnancy
Rats
Rats, Wistar

Substances

Antibodies, Monoclonal