[go: up one dir, main page]
IDEAS home Printed from https://ideas.repec.org/a/adp/ijcsmb/v6y2019i2p40-45.html
   My bibliography  Save this article

Generation of Vascular Networks from Adipocytes In Vitro

Author

Listed:
  • Ana M Blázquez Medela
  • Ashley Penton

    (Division of Cardiology, David Geffen School of Medicine at UCLA, USA)

  • Kristina I Bostrom
  • Arman Saparov
  • Medet Jumabay

    (Department of Medicine, Nazarbayev University School of Medicine, Kazakhstans)

Abstract
Multipotent cells derived from white mature adipocytes, referred to as dedifferentiated fat (DFAT) cells have the capacity differentiate into endothelial cells. The objective of this study was to modify the isolation method for DFAT cells in order to optimize the endothelial lineage potential. The adipocytes were preincubated for 24 hours, washed, and then incubated for 5 days to allow the generated DFAT cells to remain in proximity to the adipocytes while the cells aggregated into cell clusters. The DFAT cells rapidly differentiated into adipocytes after which endothelial-like cells (ECs) emerged and formed tube-like structure closely associated with the newly differentiated adipocytes. The lipid-filled cells then gradually disappeared whereas the network of tube structure expanded over the course of 3 weeks. ECs accounted for 35-45% of the cells derived from the DFAT cells, as assessed by qPCR, immunofluorescence and fluorescence-activated cell sorting. The DFAT cell-derived ECs could also be further enriched by magnetic sorting, thereby serving as a mouse cell line for further research.

Suggested Citation

  • Ana M Blázquez Medela & Ashley Penton & Kristina I Bostrom & Arman Saparov & Medet Jumabay, 2019. "Generation of Vascular Networks from Adipocytes In Vitro," International Journal of Cell Science & Molecular Biology, Juniper Publishers Inc., vol. 6(2), pages 40-45, August.
    • Handle: RePEc:adp:ijcsmb:v:6:y:2019:i:2:p:40-45
    DOI: 10.19080/IJCSMB.2019.06.555684
    as

    Download full text from publisher

    File URL: https://juniperpublishers.com/ijcsmb/pdf/IJCSMB.MS.ID.555684.pdf
    Download Restriction: no
    File URL: https://juniperpublishers.com/ijcsmb/IJCSMB.MS.ID.555684.php
    Download Restriction: no
    File URL: https://libkey.io/10.19080/IJCSMB.2019.06.555684?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:adp:ijcsmb:v:6:y:2019:i:2:p:40-45. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Robert Thomas (email available below). General contact details of provider: .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.