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Molecular Characterization of Protease Producing Stenotrophomonas and Their Cytotoxic Effects on Cancer Cell Lines

Author

Listed:
  • Anamika Thakur
  • Sanju Kumari
  • Utkarshini Sharma
  • Rohit Krishna
  • Kanak Sinha
  • Santosh Kumar

    (Department of Botany, B R A Bihar University, India)

Abstract
Proteases are highly required where proteins need some sort of alteration either structurally or functionally. They are widely used in household laundry, paper and leather industries as well as in several pharmaceutical compounds. Thus, the present study was confined to screen more than fifty isolates of microbes from the rhizospheres of fruit orchard for proteolytic activity on skimmed milk agar medium. Isolates SK701 and SK709 were found producing halo regions around the streaked mycelia. However, only isolate SK709 displayed maximum 64.6 U/ml protease activities in starch casein broth after 96 hours of culture at optimum conditions of 35°C and pH 7.6. It was identified as a gram-negative bacterium on the basis of staining, morphological, cultural, biochemical and physiological characteristics.

Suggested Citation

  • Anamika Thakur & Sanju Kumari & Utkarshini Sharma & Rohit Krishna & Kanak Sinha & Santosh Kumar, 2018. "Molecular Characterization of Protease Producing Stenotrophomonas and Their Cytotoxic Effects on Cancer Cell Lines," International Journal of Cell Science & Molecular Biology, Juniper Publishers Inc., vol. 5(1), pages 49-56, October.
    • Handle: RePEc:adp:ijcsmb:v:5:y:2018:i:1:p:49-56
    DOI: 10.19080/IJCSMB.2018.04.555660
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