The 2000 Ukrainian Amateur Cup was the fifth annual season of Ukraine's football knockout competition for amateur football teams. The competition started on 2 July 2000 and concluded on 15 October 2000.
SC Perechyn previously played under the name of Lisnyk Perechyn
Five regions that were represented last season, chose not to participate in the competition among which are such oblasts Ivano-Frankivsk, Odesa, Poltava, Sumy, and Ternopil.