S Wikipedije, slobodne enciklopedije
Član B porodice sa sličnošću sekvence 73 , znan i kao FAM73B , jest ljudski gen .[ 5]
Dužina polipeptidnog lanca je 593 aminokiseline , а molekulska težina 65.531 Da .[ 6]
10 20 30 40 50 MAFRRAEGTS MIQALAMTVA EIPVFLYTTF GQSAFSQLRL TPGLRKVLFA TALGTVALAL AAHQLKRRRR RKKQVGPEMG GEQLGTVPLP ILLARKVPSV KKGYSSRRVQ SPSSKSNDTL SGISSIEPSK HSGSSHSVAS MMAVNSSSPT AACSGLWDAR GMEESLTTSD GNAESLYMQG MELFEEALQK WEQALSVGQR GDSGSTPMPR DGLRNPETAS EPLSEPESQR KEFAEKLESL LHRAYHLQEE FGSTFPADSM LLDLERTLML PLTEGSLRLR ADDEDSLTSE DSFFSATELF ESLQTGDYPI PLSRPAAAYE EALQLVKEGR VPCRTLRTEL LGCYSDQDFL AKLHCVRQAF EGLLEDKSNQ LFFGKVGRQM VTGLMTKAEK SPKGFLESYE EMLSYALRPE TWATTRLELE GRGVVCMSFF DIVLDFILMD AFEDLENPPA SVLAVLRNRW LSDSFKETAL ATACWSVLKA KRRLLMVPDG FISHFYSVSE HVSPVLAFGF LGPKPQLAEV CAFFKHQIVQ YLRDMFDLDN VRYTSLPALA DDILQLSRRR SEILLGYLGV PAASSAGVNG ALPRENGPLG ELQ
U izbedenoj studiji proučavana je i funkcija FAM73B kod modelnih organizama . Uvjetna liniza nokaut-miševa , zvana Fam73btm1a(KOMP)Wtsi [ 15] [ 16] Međunarodnog konzorcija za nokaut-miševe — visokoprohodnog projekta za mutagenezu koji generira i distribuira modele životinjskih bolesti zainteresiranim naučnicima.[ 17] [ 18] [ 19]
Mužjaci i ženke podvrgnuti su standardiziranom fenotipskom pregledu kako bi se utvrdili efekti delecija .[ 13] [ 20] mutantnim miševima provedena su 24 ispitivanja i uočeno je šest značajnih abnormalnosti. Homozigotne mutirane životinje imale su smanjenu tjelesnu težinu, promijenjeni sastav tijela, abnormalnu morfologiju zuba, hipoalbuminemiju , smanjeni sadržaj i snagu koštanih minerala i povećanu osjetljivost na bakterijsku infekciju .[ 13]
^ a b c GRCh38: Ensembl release 89: ENSG00000148343 - Ensembl , maj 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026858 - Ensembl , maj 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Entrez Gene: family with sequence similarity 73, member B" . Pristupljeno 30. 8. 2011 .^ "UniProt, Q7L4E1" . Pristupljeno 8. 9. 2021 .^ "Body weight data for Fam73b" . Wellcome Trust Sanger Institute.^ "DEXA data for Fam73b" . Wellcome Trust Sanger Institute.^ "Radiography data for Fam73b" . Wellcome Trust Sanger Institute.^ "Clinical chemistry data for Fam73b" . Wellcome Trust Sanger Institute.^ "Salmonella infection data for Fam73b" . Wellcome Trust Sanger Institute.^ "Citrobacter infection data for Fam73b" . Wellcome Trust Sanger Institute.^ a b c Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica . 88 : 925–7. doi :10.1111/j.1755-3768.2010.4142.x . S2CID 85911512 . ^ Mouse Resources Portal , Wellcome Trust Sanger Institute.^ "International Knockout Mouse Consortium" . Arhivirano s originala , 29. 5. 2012. Pristupljeno 8. 9. 2021 .^ "Mouse Genome Informatics" .^ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function" . Nature . 474 (7351): 337–342. doi :10.1038/nature10163 . PMC 3572410 PMID 21677750 . ^ Dolgin E (2011). "Mouse library set to be knockout" . Nature . 474 (7351): 262–3. doi :10.1038/474262a PMID 21677718 . ^ Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons" . Cell . 128 (1): 9–13. doi :10.1016/j.cell.2006.12.018 PMID 17218247 . S2CID 18872015 . ^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism" . Genome Biol . 12 (6): 224. doi :10.1186/gb-2011-12-6-224 . PMC 3218837 PMID 21722353 .
Otsuki T, Ota T, Nishikawa T, et al. (2005). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries" . DNA Res . 12 (2): 117–26. doi :10.1093/dnares/12.2.117 PMID 16303743 . Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5. doi :10.1038/ng1285 PMID 14702039 . 
