High-level production of uniformly ¹⁵N- and ¹³C-enriched fusion proteins in Escherichia coli. (Q54593173)

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English	High-level production of uniformly ¹⁵N- and ¹³C-enriched fusion proteins in Escherichia coli.	scientific article

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Europe PubMed Central

PubMed publication ID

8616269

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1 June 2018

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http://europepmc.org/abstract/MED/8616269

title

High-level production of uniformly ¹⁵N- and ¹³C-enriched fusion proteins in Escherichia coli. (English)

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PubMed publication ID

8616269

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1 June 2018

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main subject

Escherichia coli

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author

Björn Nilsson

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6

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B Nilsson

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8616269

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M Jansson

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1

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8616269

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Y C Li

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2

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8616269

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L Jendeberg

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3

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8616269

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S Anderson

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4

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8616269

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G T Montelione

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5

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8616269

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publication date

1 March 1996

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8616269

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published in

Journal of Biomolecular NMR

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PubMed publication ID

8616269

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http://europepmc.org/abstract/MED/8616269

volume

7

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PubMed publication ID

8616269

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1 June 2018

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http://europepmc.org/abstract/MED/8616269

issue

2

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PubMed publication ID

8616269

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1 June 2018

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http://europepmc.org/abstract/MED/8616269

page(s)

131-141

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PubMed publication ID

8616269

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cites work

Gene regulation at the right operator (OR) bacteriophage lambda. I. OR3 and autogenous negative control by repressor

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PubMed

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Intracellular expression of BPTI fusion proteins and single column cleavage/affinity purification by chymotrypsin

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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Prospects for NMR of large proteins

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12 December 2020

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A novel isotope labeling protocol for bacterially expressed proteins

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Fusions to staphylococcal protein A

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12 December 2020

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Solution structure of a calmodulin-target peptide complex by multidimensional NMR

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ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification

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Enhanced in vitro refolding of insulin-like growth factor I using a solubilizing fusion partner

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Structures of larger proteins in solution: three- and four-dimensional heteronuclear NMR spectroscopy

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Sequence-specific assignments of the backbone 1H, 13C, and 15N resonances of the MutT enzyme by heteronuclear multidimensional NMR.

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A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin

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Separation and characterization of modified variants of recombinant human insulin-like growth factor I derived from a fusion protein secreted from Escherichia coli

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Side chain and backbone assignments in isotopically labeled proteins from two heteronuclear triple resonance experiments

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PubMed

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Expression and purification of recombinant insulin-like growth factors from Escherichia coli

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PubMed

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Uniform 13C isotope labeling of proteins with sodium acetate for NMR studies: application to human carbonic anhydrase II

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Identification and localization of bound internal water in the solution structure of interleukin 1 beta by heteronuclear three-dimensional 1H rotating-frame Overhauser 15N-1H multiple quantum coherence NMR spectroscopy.

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Expression as trpE fusion.

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Dual affinity fusion approach and its use to express recombinant human insulin-like growth factor II

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Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease

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Expression of human insulin-like growth factor I in bacteria: use of optimized gene fusion vectors to facilitate protein purification.

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Secondary and tertiary structural effects on protein NMR chemical shifts: an ab initio approach

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Chemical synthesis of insulin-like growth factor II

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A completely automated system for on-line monitoring of the production of a growth factor secreted during fermentation of Escherichia coli

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Thermodynamics of BPTI folding

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Mapping of the spectral densities of N-H bond motions in eglin c using heteronuclear relaxation experiments

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A synthetic IgG-binding domain based on staphylococcal protein A.

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An improved strategy for determining resonance assignments for isotopically enriched proteins and its application to an engineered domain of staphylococcal protein A

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NMR chemical shifts and structure refinement in proteins

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Characterization of an extended form of recombinant human insulin-like growth factor II.

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Young Investigator Award Lecture. Structures of larger proteins, protein-ligand and protein-DNA complexes by multidimensional heteronuclear NMR.

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Isotope-edited NMR of cyclosporin A bound to cyclophilin: evidence for a trans 9,10 amide bond.

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Denaturant-dependent folding of bovine pancreatic trypsin inhibitor mutants with two intact disulfide bonds

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Mutational analysis of the interaction between staphylococcal protein A and human IgG1.

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Nuclear magnetic resonance spectroscopy of a DNA complex with the uniformly 13C-labeled Antennapedia homeodomain and structure determination of the DNA-bound homeodomain.

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A novel outer-membrane-associated protease in Escherichia coli

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Plasmid vector pBR322 and its special-purpose derivatives--a review

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Structural characterization of the FK506 binding protein unfolded in urea and guanidine hydrochloride

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Four-dimensional heteronuclear triple-resonance NMR spectroscopy of interleukin-1 beta in solution

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NMR studies of [U-13C]cyclosporin A bound to cyclophilin: bound conformation and portions of cyclosporin involved in binding

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Identifiers

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10.1007/BF00203823

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PubMed publication ID

8616269

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1 June 2018

reference URL

http://europepmc.org/abstract/MED/8616269

PubMed publication ID

8616269

1 reference

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Europe PubMed Central

PubMed publication ID

8616269

retrieved

1 June 2018

reference URL

http://europepmc.org/abstract/MED/8616269

 

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