Large scale purification of detergent-soluble P-glycoprotein from Pichia pastoris cells and characterization of nucleotide binding properties of wild-type, Walker A, and Walker B mutant proteins. (Q54071315)

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English	Large scale purification of detergent-soluble P-glycoprotein from Pichia pastoris cells and characterization of nucleotide binding properties of wild-type, Walker A, and Walker B mutant proteins.	scientific article

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scholarly article

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Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

Large scale purification of detergent-soluble P-glycoprotein from Pichia pastoris cells and characterization of nucleotide binding properties of wild-type, Walker A, and Walker B mutant proteins. (English)

1 reference

Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

Pichia pastoris

object named as

Pichia pastoris

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based on heuristic

inferred from title

author name string

N Lerner-Marmarosh

1

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Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

K Gimi

2

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Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

I L Urbatsch

3

1 reference

Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

P Gros

4

1 reference

Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

A E Senior

5

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Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

language of work or name

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publication date

1 December 1999

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Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

Journal of Biological Chemistry

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Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

274

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Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

49

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Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

34711-34718

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Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

Biochemistry of multidrug resistance mediated by the multidrug transporter

1 reference

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21 January 2018

Using purified P-glycoprotein to understand multidrug resistance.

1 reference

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21 January 2018

The P-Glycoprotein Efflux Pump: How Does it Transport Drugs?

1 reference

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21 January 2018

P-glycoprotein Is Stably Inhibited by Vanadate-induced Trapping of Nucleotide at a Single Catalytic Site

1 reference

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21 January 2018

P-glycoprotein shows strong catalytic cooperativity between the two nucleotide sites.

1 reference

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21 January 2018

The catalytic cycle of P-glycoprotein

1 reference

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21 January 2018

Mutations in either nucleotide-binding site of P-glycoprotein (Mdr3) prevent vanadate trapping of nucleotide at both sites.

1 reference

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21 January 2018

Evidence for two nonidentical drug-interaction sites in the human P-glycoprotein

1 reference

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21 January 2018

Human P-glycoprotein exhibits reduced affinity for substrates during a catalytic transition state

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21 January 2018

Mechanism of action of human P-glycoprotein ATPase activity. Photochemical cleavage during a catalytic transition state using orthovanadate reveals cross-talk between the two ATP sites

1 reference

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21 January 2018

Contribution to substrate specificity and transport of nonconserved residues in transmembrane domain 12 of human P-glycoprotein

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Identification of the cyclosporin-binding site in P-glycoprotein

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21 January 2018

Structure of the multidrug resistance P-glycoprotein to 2.5 nm resolution determined by electron microscopy and image analysis.

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21 January 2018

Pleiotropic phenotype of colchicine-resistant CHO cells: cross-resistance and collateral sensitivity

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21 January 2018

Characterization of the ATPase activity of purified Chinese hamster P-glycoprotein

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21 January 2018

Covalent modification of human P-glycoprotein mutants containing a single cysteine in either nucleotide-binding fold abolishes drug-stimulated ATPase activity

1 reference

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21 January 2018

Discrete mutations introduced in the predicted nucleotide-binding sites of the mdr1 gene abolish its ability to confer multidrug resistance

1 reference

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21 January 2018

Structural flexibility of the linker region of human P-glycoprotein permits ATP hydrolysis and drug transport

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21 January 2018

Rapid purification of human P-glycoprotein mutants expressed transiently in HEK 293 cells by nickel-chelate chromatography and characterization of their drug-stimulated ATPase activities.

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21 January 2018

Purification of functional human P-glycoprotein expressed in Saccharomyces cerevisiae

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21 January 2018

Mutations in the nucleotide-binding sites of P-glycoprotein that affect substrate specificity modulate substrate-induced adenosine triphosphatase activity

1 reference

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21 January 2018

Measurement of protein using bicinchoninic acid

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21 January 2018

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

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21 January 2018

ATP hydrolysis by multidrug-resistance protein from Chinese hamster ovary cells

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21 January 2018

Binding and hydrolysis of TNP-ATP by Escherichia coli F1-ATPase.

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21 January 2018

Site-directed fluorescence labeling of P-glycoprotein on cysteine residues in the nucleotide binding domains

1 reference

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21 January 2018

The vacuolar H+-ATPase of clathrin-coated vesicles is reversibly inhibited by S-nitrosoglutathione

1 reference

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21 January 2018

Crystal structure of the ATP-binding subunit of an ABC transporter

1 reference

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21 January 2018

Identification of Residues in the Drug-binding Site of Human P-glycoprotein Using a Thiol-reactive Substrate

1 reference

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21 January 2018

Identifiers

10.1074/JBC.274.49.34711

1 reference

Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

24 May 2018

http://europepmc.org/abstract/MED/10574938

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