Contribution of active site residues to the activity and thermal stability of ribonuclease Sa (Q43078051)

13 November 2017

Contribution of active site residues to the activity and thermal stability of ribonuclease Sa (English)

1 reference

site-directed mutagenesis

12 March 2024

main subject

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

12 March 2024

1 reference

12 March 2024

Vladimir A. Mitkevich

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

12 March 2024

Saul Trevino

1 reference

13 November 2017

Stephanie Newsom

1 reference

13 November 2017

Gennady I. Yakovlev

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

12 March 2024

Kevin L. Shaw

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

12 March 2024

C. Nick Pace

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

12 March 2024

Alexander A. Makarov

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

12 March 2024

language of work or name

English

1 reference

National Center for Biotechnology Information

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=14500895

12 March 2024

publication date

1 October 2003

1 reference

full work available at URL

13 November 2017

https://europepmc.org/articles/pmc2366910?pdf=render

file format

Portable Document Format

online access status

open access

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

https://europepmc.org/articles/PMC2366910

12 March 2024

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

https://europepmc.org/articles/PMC2366910?pdf=render

12 March 2024

file format

Portable Document Format

online access status

open access

content deliverer

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1110%2Fps.03176803

12 March 2024

1 reference

https://onlinelibrary.wiley.com/doi/pdf/10.1110/ps.03176803

12 March 2024

1 reference

12 March 2024

1 reference

13 November 2017

1 reference

13 November 2017

1 reference

13 November 2017

2367-2373

1 reference

pK values of histidine residues in ribonuclease Sa: effect of salt and net charge.

13 November 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Impact of enzyme motion on activity

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Kinetic studies of guanine recognition and a phosphate group subsite on ribonuclease T1 using substitution mutants at Glu46 and Lys41.

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Structural bases of stability-function tradeoffs in enzymes

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Atomic resolution data reveal flexibility in the structure of RNase Sa

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Prediction of functionally important residues based solely on the computed energetics of protein structure.

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Tyrosine hydrogen bonds make a large contribution to protein stability

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Solution structure and dynamics of ribonuclease Sa

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

The effect of net charge on the solubility, activity, and stability of ribonuclease Sa

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Polar group burial contributes more to protein stability than nonpolar group burial

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Buried charged surface in proteins

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Charge-charge interactions influence the denatured state ensemble and contribute to protein stability

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Binding of bisubstrate analog promotes large structural changes in the unregulated catalytic trimer of aspartate transcarbamoylase: Implications for allosteric regulation

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Increasing protein stability by altering long-range coulombic interactions

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Contribution of a conserved asparagine to the conformational stability of ribonucleases Sa, Ba, and T1

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

A Decade of Protein Engineering on Ribonuclease T1— Atomic Dissection of the Enzyme‐Substrate Interactions

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Complex of ribonuclease Sa with a cyclic nucleotide and a proposed model for the reaction intermediate

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

A relationship between protein stability and protein function

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

The role of Glu-60 in the specificity of the recombinant ribonuclease from Bacillus amyloliquefaciens (barnase) towards dinucleotides, poly(A) and RNA

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

The interpretation of protein structures: Total volume, group volume distributions and packing density

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Quantitative analysis of the contribution of Glu46 and Asn98 to the guanosine specificity of ribonuclease T1.

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

His92Ala mutation in ribonuclease T1 induces segmental flexibility. An X-ray study

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Energetics of enzyme catalysis

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Subsites and catalytic mechanism of ribonuclease T1: kinetic studies using GpA, GpC, GpG, and GpU as substrates

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Subsites and catalytic mechanism of ribonuclease T1: Kinetic studies using GpC and GpU as substrates

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Expression of Bacillus amyloliquefaciens extracellular ribonuclease (barnase) in Escherichia coli following an inactivating mutation.

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Charge-charge interactions are key determinants of the pK values of ionizable groups in ribonuclease Sa (pI=3.5) and a basic variant (pI=10.2).

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Analysis of catalytic residues in enzyme active sites

15 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Electrostatic origin of the catalytic power of enzymes and the role of preorganized active sites

15 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Conformational stability and thermodynamics of folding of ribonucleases Sa, Sa2 and Sa3.

15 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Functional Interactions among the His40, Glu58 and His92 Catalysts of Ribonuclease T1 as Studied by Double and Triple Mutants

15 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

The number of cooperative regions (energetic domains) in a pepsin molecule depends on the pH of the medium

1 December 2018

1 reference

Crossref

https://api.crossref.org/works/10.1110%2FPS.03176803

Determination and restrained least-squares refinement of the structures of ribonuclease Sa and its complex with 3'-guanylic acid at 1.8 Å resolution

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1110%2FPS.03176803

21 January 2018

1 reference

https://api.crossref.org/works/10.1110%2FPS.03176803

A comparative study on the catalytic properties of guanyl-specific ribonucleases

21 January 2018

1 reference

12 December 2020

Effect of active site residues in barnase on activity and stability

1 reference

12 December 2020

PHYSICAL AND CHEMICAL CHARACTERIZATION OF THE ORDERED COMPLEXES FORMED BETWEEN POLYINOSINIC ACID, POLYCYTIDYLIC ACID AND THEIR DEOXYRIBO-ANALOGUES

1 reference

12 December 2020

Ribonuclease from Streptomyces aureofaciens at atomic resolution

1 reference

12 December 2020

Purification of Ribonucleases Sa, Sa2, and Sa3 after Expression inEscherichia coli

1 reference

12 December 2020

Contribution of arginine-82 and arginine-86 to catalysis of RNases from Bacillus intermedius (binase)

1 reference

12 December 2020

Specific protein-nucleic acid recognition in ribonuclease T1–2′-guanylic acid complex: an X-ray study

1 reference

12 December 2020

The structural and sequence homology of a family of microbial ribonucleases

1 reference

Ribonuclease T1: Structure, Function, and Stability

12 March 2024

1 reference

Cloning, sequencing and transcription of an inactivated copy of Bacillus amyloliquefaciens extracellular ribonuclease (barnase).

12 March 2024

1 reference

12 March 2024

Identifiers

DOI

10.1110/PS.03176803

1 reference

13 November 2017

1 reference

13 November 2017

1 reference