Fluorescently labelled guanine nucleotide binding proteins to analyse elementary steps of GAP-catalysed reactions (Q44235321)

1 December 2017

title

Fluorescently labelled guanine nucleotide binding proteins to analyse elementary steps of GAP-catalysed reactions (English)

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1 December 2017

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1 December 2017

Alfred Wittinghofer

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1 December 2017

Astrid Kraemer

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1 December 2017

Thilo Brinkmann

series ordinal

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1 December 2017

Ina Plettner

series ordinal

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1 December 2017

language of work or name

English

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publication date

1 December 2002

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Journal of Molecular Biology

1 December 2017

published in

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1 December 2017

volume

324

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1 December 2017

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1 December 2017

page(s)

763-774

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The guanine nucleotide-binding switch in three dimensions

1 December 2017

cites work

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

The GTPase superfamily: a conserved switch for diverse cell functions

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

The GTPase superfamily: conserved structure and molecular mechanism

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

The Ras-RasGAP Complex: Structural Basis for GTPase Activation and Its Loss in Oncogenic Ras Mutants

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Biological and biochemical properties of human rasH genes mutated at codon 61.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Formation of a Transition-State Analog of the Ras GTPase Reaction by Ras GDP, Tetrafluoroaluminate, and GTPase-Activating Proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Aluminum fluoride associates with the small guanine nucleotide binding proteins.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

The importance of two conserved arginine residues for catalysis by the ras GTPase-activating protein, neurofibromin

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Structure at 1.65 A of RhoA and its GTPase-activating protein in complex with a transition-state analogue

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Biochemical studies of the mechanism of action of the Cdc42-GTPase-activating protein

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

The conserved arginine in rho-GTPase-activating protein is essential for efficient catalysis but not for complex formation with Rho.GDP and aluminum fluoride

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Confirmation of the arginine-finger hypothesis for the GAP-stimulated GTP-hydrolysis reaction of Ras

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Identification of the catalytic domains and their functionally critical arginine residues of two yeast GTPase-activating proteins specific for Ypt/Rab transport GTPases

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

RanGAP mediates GTP hydrolysis without an arginine finger

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Rap-specific GTPase activating protein follows an alternative mechanism

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Is there a rate-limiting step before GTP cleavage by H-ras p21?

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Mechanism of GTP hydrolysis by p21N-ras catalyzed by GAP: studies with a fluorescent GTP analogue

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Individual Rate Constants for the Interaction of Ras Proteins with GTPase-Activating Proteins Determined by Fluorescence Spectroscopy

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Mutational and kinetic analyses of the GTPase-activating protein (GAP)-p21 interaction: the C-terminal domain of GAP is not sufficient for full activity

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Kinetics of inorganic phosphate release during the interaction of p21ras with the GTPase-activating proteins, p120-GAP and neurofibromin.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Monitoring the GAP catalyzed H-Ras GTPase reaction at atomic resolution in real time

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Fluorescence assays of Cdc42 interactions with target/effector proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

NTF2 mediates nuclear import of Ran.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Fluorescence methods for monitoring interactions of Rab proteins with nucleotides, Rab escort protein, and geranylgeranyltransferase

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

A molecular redox switch on p21(ras). Structural basis for the nitric oxide-p21(ras) interaction

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Aluminofluoride and beryllofluoride complexes: a new phosphate analogs in enzymology

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

BeF(3)(-) acts as a phosphate analog in proteins phosphorylated on aspartate: structure of a BeF(3)(-) complex with phosphoserine phosphatase

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

X-ray structures of the myosin motor domain of Dictyostelium discoideum complexed with MgADP.BeFx and MgADP.AlF4-

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Structure-based mutagenesis reveals distinct functions for Ras switch 1 and switch 2 in Sos-catalyzed guanine nucleotide exchange

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Differential structural requirements for interaction of Ras protein with its distinct downstream effectors.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

ras effector loop mutations that dissociate p120GAP and neurofibromin interactions

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Site-directed mutagenesis, fluorescence, and two-dimensional NMR studies on microenvironments of effector region aromatic residues of human c-Ha-Ras protein

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

The role of the metal ion in the p21ras catalysed GTP-hydrolysis: Mn2+ versus Mg2+

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Rap1 signalling: adhering to new models

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Molecular cloning of a GTPase activating protein specific for the Krev-1 protein p21rap1

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Dynamic and Equilibrium Studies on the Interaction of Ran with Its Effector, RanBP1

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Conformational transitions in p21ras and in its complexes with the effector protein Raf-RBD and the GTPase activating protein GAP.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

The Binding of Nucleotides and Metal Ions to Elongation Factor Tu fromBacillus stearothermophilus as Studied by Equilibrium Dialysis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Structures of Cdc42 bound to the active and catalytically compromised forms of Cdc42GAP

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Magnesium fluoride-dependent binding of small G proteins to their GTPase-activating proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Targeted disruption of the neurofibromatosis type-1 gene leads to developmental abnormalities in heart and various neural crest-derived tissues

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Loss of NF1 results in activation of the Ras signaling pathway and leads to aberrant growth in haematopoietic cells

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Vascular system defects and neuronal apoptosis in mice lacking ras GTPase-activating protein

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Neurofibromin GTPase-activating protein-related domains restore normal growth in Nf1-/- cells

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

The ARF1 GTPase-activating protein: zinc finger motif and Golgi complex localization

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Crystal structure of the ARF-GAP domain and ankyrin repeats of PYK2-associated protein beta

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Structural and functional analysis of the ARF1-ARFGAP complex reveals a role for coatomer in GTP hydrolysis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Differential interaction of the ras family GTP-binding proteins H-Ras, Rap1A, and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Reassessment of Ellman's reagent

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

Dynamic interaction of cAMP with the Rap guanine-nucleotide exchange factor Epac1.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2901136-1

10.1016/S0022-2836(02)01136-1

7 January 2021

Identifiers

DOI

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1 December 2017

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