A Ty1 reverse transcriptase active-site aspartate mutation blocks transposition but not polymerization (Q33842826)

29 July 2017

title

A Ty1 reverse transcriptase active-site aspartate mutation blocks transposition but not polymerization (English)

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29 July 2017

O Uzun

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29 July 2017

A Gabriel

series ordinal

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29 July 2017

publication date

1 July 2001

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29 July 2017

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29 July 2017

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29 July 2017

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29 July 2017

page(s)

6337-6347

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Expression of an active form of recombinant Ty1 reverse transcriptase in Escherichia coli: a fusion protein containing the C-terminal region of the Ty1 integrase linked to the reverse transcriptase-RNase H domain exhibits polymerase and RNase H acti

29 July 2017

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Product catalyzes the deamidation of D145N dehalogenase to produce the wild-type enzyme.

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Replication errors during in vivo Ty1 transposition are linked to heterogeneous RNase H cleavage sites

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5 July 2018

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5 July 2018

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5 July 2018

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5 July 2018

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5 July 2018

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RNase H domain of Moloney murine leukemia virus reverse transcriptase retains activity but requires the polymerase domain for specificity

5 July 2018

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5 July 2018

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Biochemical analysis of catalytically crucial aspartate mutants of human immunodeficiency virus type 1 reverse transcriptase.

5 July 2018

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Specific initiation and switch to elongation of human immunodeficiency virus type 1 reverse transcription require the post-transcriptional modifications of primer tRNA3Lys

5 July 2018

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Mechanistic implications from the structure of a catalytic fragment of Moloney murine leukemia virus reverse transcriptase

5 July 2018

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Biochemical and electron microscope analyses of the DNA reverse transcripts present in the virus-like particles of the yeast transposon Ty1. Identification of a second origin of Ty1DNA plus strand synthesis

5 July 2018

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Transcriptional activity and mutational analysis of recombinant vesicular stomatitis virus RNA polymerase.

5 July 2018

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A role for reverse transcripts in gene conversion

5 July 2018

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Expression and partial purification of enzymatically active recombinant Ty1 integrase in Saccharomyces cerevisiae

5 July 2018

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In-frame linker insertion mutagenesis of yeast transposon Ty1: phenotypic analysis.

5 July 2018

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Heterogeneous functional Ty1 elements are abundant in the Saccharomyces cerevisiae genome.

5 July 2018

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Two defective forms of reverse transcriptase can complement to restore retroviral infectivity

5 July 2018

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Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 A resolution shows bent DNA

5 July 2018

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RNase H domain mutations affect the interaction between Moloney murine leukemia virus reverse transcriptase and its primer-template

5 July 2018

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Insights into DNA polymerization mechanisms from structure and function analysis of HIV-1 reverse transcriptase

5 July 2018

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5 July 2018

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Efficient Homologous Recombination of Ty1 Element cDNA When Integration Is Blocked

5 July 2018

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5 July 2018

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5 July 2018

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A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

5 July 2018

1 reference

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Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus

5 July 2018

1 reference

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Reverse transcriptase as the major determinant for selective packaging of tRNA's into Avian sarcoma virus particles

5 July 2018

1 reference

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Rapid and efficient site-specific mutagenesis without phenotypic selection

5 July 2018

1 reference

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A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=114356

Saccharomyces cerevisiae SPT3 gene is required for transposition and transpositional recombination of chromosomal Ty elements

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=114356

Identification of four conserved motifs among the RNA-dependent polymerase encoding elements

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=114356

Infectious potential of human immunodeficiency virus type 1 reverse transcriptase mutants with altered inhibitor sensitivity

5 July 2018

1 reference

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Origins and evolutionary relationships of retroviruses

5 July 2018

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Genes encoding a subunit of respiratory NADH dehydrogenase (ND1) and a reverse transcriptase-like protein (RTL) are linked to ribosomal RNA gene pieces in Chlamydomonas reinhardtii mitochondrial DNA.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=114356

Site-specific mutagenesis of AIDS virus reverse transcriptase

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=114356

Ty element transposition: reverse transcriptase and virus-like particles

5 July 2018

1 reference

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An attempt to unify the structure of polymerases

5 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=114356

Structural basis for the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I: a two metal ion mechanism

5 July 2018

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Single-step selection for Ty1 element retrotransposition

5 July 2018

1 reference

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A constant rate of spontaneous mutation in DNA-based microbes

5 July 2018

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Reverse transcriptase encoded by a retrotransposon from the trypanosomatid Crithidia fasciculata

5 July 2018

1 reference

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A recombinant ribonuclease H domain of HIV-1 reverse transcriptase that is enzymatically active

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=114356

Subunit-selective mutagenesis indicates minimal polymerase activity in heterodimer-associated p51 HIV-1 reverse transcriptase

5 July 2018

1 reference

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Proteolytic processing of pol-TYB proteins from the yeast retrotransposon Ty1

5 July 2018

1 reference

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Retroelements in bacteria

5 July 2018

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Origin and evolution of retroelements based upon their reverse transcriptase sequences

5 July 2018

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Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase

5 July 2018

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RNA-mediated recombination in S. cerevisiae

5 July 2018

1 reference

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Crystal structure at 3.5 A resolution of HIV-1 reverse transcriptase complexed with an inhibitor

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=114356

Involvement of cDNA in homologous recombination between Ty elements in Saccharomyces cerevisiae

5 July 2018

1 reference

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Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1

5 July 2018

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Initiator methionine tRNA is essential for Ty1 transposition in yeast

5 July 2018

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Genomic analysis of Caenorhabditis elegans reveals ancient families of retroviral-like elements

5 July 2018

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Telomerase and Retrotransposons: Which Came First?

29 October 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=114356

Mutational analysis of the fingers and palm subdomains of human immunodeficiency virus type-1 (HIV-1) reverse transcriptase

29 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=114356

The DNA intermediate in yeast Ty1 element transposition copurifies with virus-like particles: cell-free Ty1 transposition

29 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=114356

Contacts between Reverse Transcriptase and the Primer Strand Govern the Transition from Initiation to Elongation of HIV-1 Reverse Transcription

29 October 2018

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12 December 2020

A general method for the chromosomal amplification of genes in yeast

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12 December 2020

The Mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor

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12 December 2020

Evidence for a “non-genetic” origin of the A1 chains of α-crystallin

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12 December 2020

Ty elements transpose through an RNA intermediate

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12 December 2020

Characterization of products of TY1-mediated reverse transcription in Saccharomyces cerevisiae

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12 December 2020

Activation of an Asp-124-->Asn mutant of haloalkane dehalogenase by hydrolytic deamidation of asparagine

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12 December 2020

Group II intron mobility occurs by target DNA-primed reverse transcription

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12 December 2020

Restoration of tRNA3Lys-primed(-)-strand DNA synthesis to an HIV-1 reverse transcriptase mutant with extended tRNAs. Implications for retroviral replication

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12 December 2020

Mutations within the primer grip region of HIV-1 reverse transcriptase result in loss of RNase H function

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12 December 2020

10.1128/JVI.75.14.6337-6347.2001

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DOI

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29 July 2017

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29 July 2017

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