Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution (Q33214817)

24 July 2017

title

Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution (English)

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24 July 2017

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Manfred T Reetz

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24 July 2017

Thorsten Eggert

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24 July 2017

Susanne Aileen Funke

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24 July 2017

Nalam M Rao

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24 July 2017

Priyamvada Acharya

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24 July 2017

Holger Krumm

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24 July 2017

Karl-Erich Jaeger

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24 July 2017

publication date

1 June 2005

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24 July 2017

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24 July 2017

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24 July 2017

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24 July 2017

page(s)

1062-1067

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Enantioselective biocatalysis optimized by directed evolution

24 July 2017

cites work

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Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications

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Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution

21 January 2018

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Random-priming in vitro recombination: An effective tool for directed evolution

21 January 2018

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Recombination and chimeragenesis by in vitro heteroduplex formation and in vivo repair

21 January 2018

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DNA shuffling method for generating highly recombined genes and evolved enzymes

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Construction of DNA-shuffled and incrementally truncated libraries by a mutagenic and unidirectional reassembly method: changing from a substrate specificity of phospholipase to that of lipase

21 January 2018

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Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently

21 January 2018

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Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution

21 January 2018

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Random DNA fragmentation with endonuclease V: application to DNA shuffling

21 January 2018

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High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2

21 January 2018

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DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

21 January 2018

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Rapid evolution of a protein in vitro by DNA shuffling

21 January 2018

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Phage display as a tool for the directed evolution of enzymes

21 January 2018

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Ultra-high-throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts

21 January 2018

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Optimization of DNA shuffling for high fidelity recombination

21 January 2018

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Improved method for PCR-mediated site-directed mutagenesis

21 January 2018

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A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase

21 January 2018

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A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR

21 January 2018

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Structural basis of selection and thermostability of laboratory evolved Bacillus subtilis lipase

21 January 2018

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Directed Evolution of an EnantioselectiveBacillus subtilisLipase

21 January 2018

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21 January 2018

Identifiers

DOI

10.1002/CBIC.200400417

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24 July 2017

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