Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h. (Q33209678)

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scientific article published on 7 December 2004

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English	Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.	scientific article published on 7 December 2004

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scholarly article

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Europe PubMed Central

PubMed publication ID

15585660

retrieved

23 July 2017

title

Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h. (English)

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stated in

Europe PubMed Central

PubMed publication ID

15585660

retrieved

23 July 2017

author name string

Joyce Chiu

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1

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Europe PubMed Central

PubMed publication ID

15585660

retrieved

23 July 2017

Paul E March

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2

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stated in

Europe PubMed Central

PubMed publication ID

15585660

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23 July 2017

Ryan Lee

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3

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stated in

Europe PubMed Central

PubMed publication ID

15585660

retrieved

23 July 2017

Daniel Tillett

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4

1 reference

stated in

Europe PubMed Central

PubMed publication ID

15585660

retrieved

23 July 2017

language of work or name

English

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publication date

7 December 2004

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stated in

Europe PubMed Central

PubMed publication ID

15585660

retrieved

23 July 2017

published in

Nucleic Acids Research

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stated in

Europe PubMed Central

PubMed publication ID

15585660

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23 July 2017

volume

32

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Europe PubMed Central

PubMed publication ID

15585660

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23 July 2017

issue

21

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Europe PubMed Central

PubMed publication ID

15585660

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23 July 2017

page(s)

e174

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Europe PubMed Central

PubMed publication ID

15585660

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23 July 2017

cites work

Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites

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21 June 2018

An efficient 1-tube PCR method for internal site-directed mutagenesis of large amplified molecules

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PubMed Central

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21 June 2018

Rapid and efficient site-specific mutagenesis without phenotypic selection

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PubMed Central

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21 June 2018

Construction of mutant and chimeric genes using the polymerase chain reaction

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21 June 2018

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

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21 June 2018

A simple method for site-directed mutagenesis using the polymerase chain reaction

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PubMed Central

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

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21 June 2018

Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).

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21 June 2018

High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis

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21 June 2018

Receptor and antibody epitopes in human growth hormone identified by homolog-scanning mutagenesis.

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21 June 2018

The "megaprimer" method of site-directed mutagenesis

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21 June 2018

A general method for rapid site-directed mutagenesis using the polymerase chain reaction.

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PubMed Central

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21 June 2018

Site-directed mutagenesis using a double-stranded DNA fragment as a PCR primer

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21 June 2018

Site-directed mutagenesis of virtually any plasmid by eliminating a unique site

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21 June 2018

Enzymatic inverse PCR: a restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis

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21 June 2018

PCR-based random mutagenesis method using spiked oligonucleotides to randomize selected parts of a gene without any wild-type background

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31 October 2018

Site-Directed Mutagenesis: A Two-Step Method Using PCR and Dpn I

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15585660

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Generation of multiple site-specific mutations in a single polymerase chain reaction product

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15585660

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Inverse PCR-generated internally deleted constructs for direct characterization of promoter regulatory regions

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15585660

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site-directed mutagenesis

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15585660

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15585660

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

DOI

10.1093/NAR/GNH172

1 reference

stated in

Europe PubMed Central

PubMed publication ID

15585660

retrieved

23 July 2017

PMC publication ID

535700

1 reference

stated in

Europe PubMed Central

PubMed publication ID

15585660

retrieved

23 July 2017

PubMed publication ID

15585660

1 reference

stated in

Europe PubMed Central

PubMed publication ID

15585660

retrieved

23 July 2017

ResearchGate publication ID

8141695

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