Why does threonine, and not serine, function as the active site nucleophile in proteasomes? (Q30870481)

27 June 2017

title

Why does threonine, and not serine, function as the active site nucleophile in proteasomes? (English)

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27 June 2017

Kisselev AF

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27 June 2017

Songyang Z

series ordinal

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27 June 2017

Goldberg AL

series ordinal

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27 June 2017

language of work or name

English

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publication date

1 May 2000

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Journal of Biological Chemistry

27 June 2017

published in

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27 June 2017

volume

275

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27 June 2017

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27 June 2017

page(s)

14831-14837

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Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules

27 June 2017

cites work

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Lactacystin and clasto-lactacystin beta-lactone modify multiple proteasome beta-subunits and inhibit intracellular protein degradation and major histocompatibility complex class I antigen presentation

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Structure and functions of the 20S and 26S proteasomes

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Ubiquitin-dependent protein degradation

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

The 26S proteasome: a molecular machine designed for controlled proteolysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

The proteasome: paradigm of a self-compartmentalizing protease

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Crystal structure of the 20S proteasome from the archaeon T. acidophilum at 3.4 A resolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Conformational constraints in protein degradation by the 20S proteasome

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Protein translocation channels in the proteasome and other proteases

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Proteolysis: The proteasome: a protein-degrading organelle?

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

An archaebacterial ATPase, homologous to ATPases in the eukaryotic 26 S proteasome, activates protein breakdown by 20 S proteasomes

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Cleavage motifs of the yeast 20S proteasome beta subunits deduced from digests of enolase 1.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Primary structure of the Thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

A protein catalytic framework with an N-terminal nucleophile is capable of self-activation

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Structure of the allosteric regulatory enzyme of purine biosynthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Histone mRNA in Xenopus laevis ovaries: identification of the H4 messenger

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Three-dimensional structure of Escherichia coli asparagine synthetase B: a short journey from substrate to product

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Effects of site-directed mutations on processing and activities of penicillin G acylase from Escherichia coli ATCC 11105

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Penicillin acylase has a single-amino-acid catalytic centre

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Three-dimensional structure of human lysosomal aspartylglucosaminidase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

HslV-HslU: A novel ATP-dependent protease complex in Escherichia coli related to the eukaryotic proteasome

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Proteasome from Thermoplasma acidophilum: a threonine protease

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

The Active Sites of the Eukaryotic 20 S Proteasome and Their Involvement in Subunit Precursor Processing

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Autocatalytic subunit processing couples active site formation in the 20S proteasome to completion of assembly

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Identification of the yeast 20S proteasome catalytic centers and subunit interactions required for active-site formation

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Contribution of proteasomal beta-subunits to the cleavage of peptide substrates analyzed with yeast mutants

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Structure of 20S proteasome from yeast at 2.4 A resolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Reactions of [14C]-3,4-dichloroisocoumarin with subunits of pituitary and spleen multicatalytic proteinase complexes (proteasomes).

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Covalent modification of the active site threonine of proteasomal beta subunits and the Escherichia coli homolog HslV by a new class of inhibitors

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

The catalytic sites of 20S proteasomes and their role in subunit maturation: a mutational and crystallographic study

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Eukaryotic 20S proteasome catalytic subunit propeptides prevent active site inactivation by N-terminal acetylation and promote particle assembly

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Structure and function of the 20S proteasome and of its regulatory complexes

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Autocatalytic processing of the 20S proteasome

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Calculation of protein extinction coefficients from amino acid sequence data

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Catalytic mechanism of the 20S proteasome of Thermoplasma acidophilum revealed by X-ray crystallography

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Mechanistic studies on the inactivation of the proteasome by lactacystin: a central role for clasto-lactacystin beta-lactone

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo antiinflammatory activity

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Recognition of the polyubiquitin proteolytic signal

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Proteasome inhibitors: valuable new tools for cell biologists

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

New insights on the specificity of penicillin acylase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Crystal structures of Flavobacterium glycosylasparaginase. An N-terminal nucleophile hydrolase activated by intramolecular proteolysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Processive degradation of proteins and other catalytic properties of the proteasome from Thermoplasma acidophilum

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Range of Sizes of Peptide Products Generated during Degradation of Different Proteins by Archaeal Proteasomes

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

The sizes of peptides generated from protein by mammalian 26 and 20 S proteasomes. Implications for understanding the degradative mechanism and antigen presentation

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Critical elements in proteasome assembly

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Proteasome β-type subunits: unequal roles of propeptides in core particle maturation and a hierarchy of active site function

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Site-directed mutagenesis of essential residues involved in the mechanism of bacterial glycosylasparaginase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Human polyomavirus BK and immunogenicity of mammalian DNA: a conceptual framework

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Proteasome active sites allosterically regulate each other, suggesting a cyclical bite-chew mechanism for protein breakdown

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

Kinetic studies of the branched chain amino acid preferring peptidase activity of the 20S proteasome: development of a continuous assay and inhibition by tripeptide aldehydes and clasto-lactacystin beta-lactone

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.275.20.14831

21 January 2018

Identifiers

DOI

10.1074/JBC.275.20.14831

1 reference

27 June 2017

1 reference