A versatile ligation-independent cloning method suitable for high-throughput expression screening applications (Q35804580)

10 August 2017

A versatile ligation-independent cloning method suitable for high-throughput expression screening applications (English)

1 reference

10 August 2017

Joanne Nettleship

0 references

David I Stuart

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Nick S Berrow

1 reference

10 August 2017

David Alderton

1 reference

10 August 2017

Sarah Sainsbury

1 reference

10 August 2017

Rene Assenberg

1 reference

10 August 2017

Nahid Rahman

1 reference

10 August 2017

Raymond J Owens

1 reference

10 August 2017

language of work or name

English

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publication date

22 February 2007

1 reference

10 August 2017

Nucleic Acids Research

1 reference

10 August 2017

1 reference

10 August 2017

1 reference

10 August 2017

e45

1 reference

A time- and cost-efficient system for high-level protein production in mammalian cells

10 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

SPINE bioinformatics and data-management aspects of high-throughput structural biology

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE.

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

The chromosome 6p22 haplotype associated with dyslexia reduces the expression of KIAA0319, a novel gene involved in neuronal migration

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Large-scale transient transfection of mammalian cells: a newly emerging attractive option for recombinant protein production

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Crystal structure of nitrogen regulatory protein IIANtr from Neisseria meningitidis.

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

RONN: the bio-basis function neural network technique applied to the detection of natively disordered regions in proteins

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

A procedure for setting up high-throughput nanolitre crystallization experiments. Crystallization workflow for initial screening, automated storage, imaging and optimization

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Transient gene expression in HEK293 cells: peptone addition posttransfection improves recombinant protein synthesis

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Rapid parallel expression in E. Coli and insect cells: analysis of five lef gene products of the Autographa californica multiple nuclear polyhedrosis virus (AcMNPV).

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

High-throughput screening for soluble recombinant expressed kinases in Escherichia coli and insect cells

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Complete Genome Sequence of Neisseria meningitidis Serogroup B Strain MC58

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Ligation-independent cloning of PCR products (LIC-PCR)

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Use of T7 RNA polymerase to direct expression of cloned genes

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Structural features in eukaryotic mRNAs that modulate the initiation of translation

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Efficient selection for high-expression transfectants with a novel eukaryotic vector

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Methods and results for semi-automated cloning using integrated robotics.

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination.

25 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes.

25 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874605

An improved method for fast, robust, and seamless integration of DNA fragments into multiple plasmids

25 September 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/17317681

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1093/NAR/GKM047

1 reference

10 August 2017

1 reference

10 August 2017

PubMed publication ID

17317681

1 reference