A spectrophotometric assay for conjugation of ubiquitin and ubiquitin-like proteins. (Q35226580)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21771579%20AND%20SRC:MED&resulttype=core&format=json

8 February 2020

title

A spectrophotometric assay for conjugation of ubiquitin and ubiquitin-like proteins (English)

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8 February 2020

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Christopher E. Berndsen

series ordinal

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8 February 2020

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8 February 2020

author name string

Christopher E Berndsen

series ordinal

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PubMed publication ID

21771579

7 August 2017

Cynthia Wolberger

series ordinal

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PubMed publication ID

21771579

7 August 2017

language of work or name

English

0 references

publication date

2 July 2011

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8 February 2020

published in

Analytical Biochemistry

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8 February 2020

volume

418

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8 February 2020

issue

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8 February 2020

page(s)

102-110

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E2s: structurally economical and functionally replete

8 February 2020

cites work

1 reference

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27 July 2018

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A Dual E3 Mechanism for Rub1 Ligation to Cdc53

27 July 2018

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Catalysis of lysine 48-specific ubiquitin chain assembly by residues in E2 and ubiquitin.

27 July 2018

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The Structure and Conformation of Lys63-Linked Tetraubiquitin

27 July 2018

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27 July 2018

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RING domain E3 ubiquitin ligases

27 July 2018

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Physiological functions of the HECT family of ubiquitin ligases

27 July 2018

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Ubiquitin-like protein activation by E1 enzymes: the apex for downstream signalling pathways

27 July 2018

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Analysis of in vitro SUMOylation using bioluminescence resonance energy transfer (BRET).

27 July 2018

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27 July 2018

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Purification of SUMO conjugating enzymes and kinetic analysis of substrate conjugation.

27 July 2018

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27 July 2018

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Quantitative analysis of multi-protein interactions using FRET: application to the SUMO pathway

27 July 2018

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Taking it step by step: mechanistic insights from structural studies of ubiquitin/ubiquitin-like protein modification pathways

27 July 2018

1 reference

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Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation

27 July 2018

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Ubiquitin: structures, functions, mechanisms

27 July 2018

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Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha

27 July 2018

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Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair

27 July 2018

1 reference

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The mechanism of ubiquitin activating enzyme. A kinetic and equilibrium analysis

27 July 2018

1 reference

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Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation

27 July 2018

1 reference

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A specific endpoint assay for ubiquitin

27 July 2018

1 reference

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Synthesis of free and proliferating cell nuclear antigen-bound polyubiquitin chains by the RING E3 ubiquitin ligase Rad5.

27 July 2018

1 reference

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A toolbox approach to high-throughput TR-FRET-based SUMOylation and DeSUMOylation assays

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178097

Breaking the chains: structure and function of the deubiquitinases

26 September 2018

1 reference

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Origin and function of ubiquitin-like proteins

26 September 2018

1 reference

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Polyubiquitin chains: functions, structures, and mechanisms

26 September 2018

1 reference

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Substrate modification with lysine 63-linked ubiquitin chains through the UBC13-UEV1A ubiquitin-conjugating enzyme

26 September 2018

1 reference

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Structure and Analysis of a Complex between SUMO and Ubc9 Illustrates Features of a Conserved E2-Ubl Interaction

26 September 2018

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A homogeneous FRET assay system for multiubiquitin chain assembly and disassembly

26 September 2018

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26 September 2018

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Purification and activity assays for Ubc9, the ubiquitin-conjugating enzyme for the small ubiquitin-like modifier SUMO.

26 September 2018

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Purification of E1 and E1-like enzymes

26 September 2018

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Ubiquitin binding site of the ubiquitin E2 variant (UEV) protein Mms2 is required for DNA damage tolerance in the yeast RAD6 pathway

26 September 2018

1 reference

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Microtiter assay for glutamine synthetase biosynthetic activity using inorganic phosphate detection

26 September 2018

1 reference

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Assays for protein-tyrosine phosphatases

26 September 2018

1 reference

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In vitro assembly and recognition of Lys-63 polyubiquitin chains

26 September 2018

1 reference

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Functional heterogeneity of ubiquitin carrier proteins

26 September 2018

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12 December 2020

Structural basis for non-covalent interaction between ubiquitin and the ubiquitin conjugating enzyme variant human MMS2

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12 December 2020

Strategy for Efficient Site-Specific FRET-Dye Labeling of Ubiquitin

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12 December 2020

The determination of inorganic phosphate in the presence of labile phosphate esters

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12 December 2020

Development of a ubiquitin transfer assay for high throughput screening by fluorescence resonance energy transfer

1 reference

12 December 2020

Steady-state kinetic analysis of human ubiquitin-activating enzyme (E1) using a fluorescently labeled ubiquitin substrate

1 reference

12 December 2020

A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase

1 reference

12 December 2020

Immunochemical analysis of the turnover of ubiquitin-protein conjugates in intact cells. Relationship to the breakdown of abnormal proteins

1 reference

12 December 2020

Ubiquitin adenylate: structure and role in ubiquitin activation

1 reference

12 December 2020

Identifiers

DOI

10.1016/J.AB.2011.06.034

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21771579%20AND%20SRC:MED&resulttype=core&format=json

8 February 2020

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21771579%20AND%20SRC:MED&resulttype=core&format=json

8 February 2020

PubMed publication ID

21771579

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21771579%20AND%20SRC:MED&resulttype=core&format=json