Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations (Q35182675)

7 August 2017

title

Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations (English)

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7 August 2017

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Ulrich Schwaneberg

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7 August 2017

Anna Joëlle Ruff

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7 August 2017

Jing Zhao

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7 August 2017

Tsvetan Kardashliev

series ordinal

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7 August 2017

Marco Bocola

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7 August 2017

publication date

14 July 2014

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Biotechnology and Bioengineering

7 August 2017

published in

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7 August 2017

volume

111

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7 August 2017

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7 August 2017

page(s)

2380-2389

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Reengineered glucose oxidase for amperometric glucose determination in diabetes analytics

7 August 2017

cites work

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Protein stability promotes evolvability

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Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution

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SeSaM-Tv-II generates a protein sequence space that is unobtainable by epPCR.

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To get what we aim for--progress in diversity generation methods

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P450 BM3 crystal structures reveal the role of the charged surface residue Lys/Arg184 in inversion of enantioselective styrene epoxidation

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Multi-site saturation by OmniChange yields a pH- and thermally improved phytase

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Directed evolution of a highly active Yersinia mollaretii phytase.

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Transversion-enriched sequence saturation mutagenesis (SeSaM-Tv+): a random mutagenesis method with consecutive nucleotide exchanges that complements the bias of error-prone PCR.

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A statistical analysis of random mutagenesis methods used for directed protein evolution.

21 January 2018

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Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution

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An efficient one-step site-directed and site-saturation mutagenesis protocol

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Directed Evolution of an EnantioselectiveBacillus subtilisLipase

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Further improvement of phosphite dehydrogenase thermostability by saturation mutagenesis

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Directed Evolution of an Enantioselective Enzyme through Combinatorial Multiple-Cassette Mutagenesis

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dRTP and dPTP a complementary nucleotide couple for the Sequence Saturation Mutagenesis (SeSaM) method

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Challenges of the genetic code for exploring sequence space in directed protein evolution

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Widening the pH activity profile of a fungal laccase by directed evolution

21 January 2018

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21 January 2018

Identifiers

DOI

10.1002/BIT.25302

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7 August 2017

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