High-throughput sequencing of 16S rRNA gene amplicons: effects of extraction procedure, primer length and annealing temperature (Q34292810)

1 August 2017

title

High-throughput sequencing of 16S rRNA gene amplicons: effects of extraction procedure, primer length and annealing temperature (English)

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high-throughput sequencing

1 August 2017

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Mark J Pallen

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Martin J Sergeant

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1 August 2017

Chrystala Constantinidou

series ordinal

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1 August 2017

Tristan Cogan

series ordinal

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1 August 2017

Charles W Penn

series ordinal

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1 August 2017

language of work or name

English

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publication date

29 May 2012

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1 August 2017

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1 August 2017

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1 August 2017

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1 August 2017

page(s)

e38094

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Creative Commons Attribution 4.0 International

1 August 2017

start time

29 May 2012

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April 2022 Public Data File from Crossref

copyrighted

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cites work

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Removing noise from pyrosequenced amplicons

6 July 2018

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Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method

6 July 2018

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Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions

6 July 2018

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Search and clustering orders of magnitude faster than BLAST

6 July 2018

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QIIME allows analysis of high-throughput community sequencing data

6 July 2018

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Comparative analysis of fecal DNA extraction methods with phylogenetic microarray: effective recovery of bacterial and archaeal DNA using mechanical cell lysis

6 July 2018

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Wrinkles in the rare biosphere: pyrosequencing errors can lead to artificial inflation of diversity estimates

6 July 2018

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6 July 2018

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Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes

6 July 2018

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6 July 2018

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6 July 2018

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MUSCLE: multiple sequence alignment with high accuracy and high throughput

6 July 2018

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Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting

6 July 2018

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Genetic diversity in Sargasso Sea bacterioplankton

6 July 2018

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Experimental factors affecting PCR-based estimates of microbial species richness and evenness.

6 July 2018

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Quantification of the detrimental effect of a single primer-template mismatch by real-time PCR using the 16S rRNA gene as an example.

21 January 2018

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Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis

21 January 2018

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https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0038094

10.1371/JOURNAL.PONE.0038094

21 January 2018

Identifiers

DOI

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1 August 2017

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1 August 2017

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