Expression, purification, and characterization of Aspergillus fumigatus sterol 14-alpha demethylase (CYP51) isoenzymes A and B. (Q34151112)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20660663%20AND%20SRC:MED&resulttype=core&format=json

8 February 2020

title

Expression, purification, and characterization of Aspergillus fumigatus sterol 14-alpha demethylase (CYP51) isoenzymes A and B (English)

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20660663%20AND%20SRC:MED&resulttype=core&format=json

14-alpha sterol demethylase

8 February 2020

main subject

Aspergillus fumigatus

0 references

1 reference

GeneDB

Putative 14-alpha demethylase with a predicted role in ergosterol biosynthesis

6 February 2019

1 reference

6 February 2019

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20660663%20AND%20SRC:MED&resulttype=core&format=json

8 February 2020

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8 February 2020

Nadja Melo

1 reference

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8 February 2020

Claire M Martel

1 reference

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8 February 2020

W David Nes

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20660663%20AND%20SRC:MED&resulttype=core&format=json

8 February 2020

Steven L Kelly

1 reference

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8 February 2020

Diane E Kelly

1 reference

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8 February 2020

language of work or name

English

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publication date

26 July 2010

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Antimicrobial Agents and Chemotherapy

8 February 2020

published in

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8 February 2020

volume

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8 February 2020

issue

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8 February 2020

page(s)

4225-4234

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20660663%20AND%20SRC:MED&resulttype=core&format=json

Azole binding properties of Candida albicans sterol 14-alpha demethylase (CaCYP51).

8 February 2020

cites work

1 reference

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Structural Characterization of CYP51 from Trypanosoma cruzi and Trypanosoma brucei Bound to the Antifungal Drugs Posaconazole and Fluconazole

28 July 2018

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Structural basis of human CYP51 inhibition by antifungal azoles

28 July 2018

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Expression and functional characterization of cytochrome P450 26A1, a retinoic acid hydroxylase

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28 July 2018

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A new Aspergillus fumigatus resistance mechanism conferring in vitro cross-resistance to azole antifungals involves a combination of cyp51A alterations.

28 July 2018

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Mistaken identity: Neosartorya pseudofischeri and its anamorph masquerading as Aspergillus fumigatus

28 July 2018

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Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drug susceptibility.

28 July 2018

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Fluconazole binding and sterol demethylation in three CYP51 isoforms indicate differences in active site topology

28 July 2018

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THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES

28 July 2018

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Mutations in Aspergillus fumigatus resulting in reduced susceptibility to posaconazole appear to be restricted to a single amino acid in the cytochrome P450 14alpha-demethylase

28 July 2018

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Intron-exon organization and phylogeny in a large superfamily, the paralogous cytochrome P450 genes of Arabidopsis thaliana.

28 July 2018

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Cytochrome P450 and the individuality of species

28 July 2018

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Itraconazole resistance in Aspergillus fumigatus

28 July 2018

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Kinetics of the reversible inhibition of enzyme-catalysed reactions by tight-binding inhibitors

28 July 2018

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Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae

28 July 2018

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A novel method for studying ergosterol biosynthesis by a cell-free preparation of Aspergillus fumigatus and its inhibition by azole antifungal agents

28 July 2018

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27 Measurement of substrate and inhibitor binding to microsomal cytochrome P-450 by optical-difference spectroscopy

28 July 2018

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The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase

28 July 2018

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Identification, characterization, and azole-binding properties of Mycobacterium smegmatis CYP164A2, a homolog of ML2088, the sole cytochrome P450 gene of Mycobacterium leprae

28 October 2018

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Does pre-exposure of Aspergillus fumigatus to voriconazole or posaconazole in vitro affect its virulence and the in vivo activity of subsequent posaconazole or voriconazole, respectively? A study in a fly model of aspergillosis

28 October 2018

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Chemosensitization prevents tolerance of Aspergillus fumigatus to antimycotic drugs

28 October 2018

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Comparison of lanosterol-14 alpha-demethylase (CYP51) of human and Candida albicans for inhibition by different antifungal azoles.

28 October 2018

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Purification and characterization of bovine steroid 21-hydroxylase (P450c21) efficiently expressed in Escherichia coli

28 October 2018

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Conservation in the CYP51 family. Role of the B' helix/BC loop and helices F and G in enzymatic function

28 October 2018

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Substrate recognition sites in 14alpha-sterol demethylase from comparative analysis of amino acid sequences and X-ray structure of Mycobacterium tuberculosis CYP51.

28 October 2018

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Identification of two different 14-alpha sterol demethylase-related genes (cyp51A and cyp51B) in Aspergillus fumigatus and other Aspergillus species

28 October 2018

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28 October 2018

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Folding requirements are different between sterol 14alpha-demethylase (CYP51) from Mycobacterium tuberculosis and human or fungal orthologs

28 October 2018

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Contribution of mutations in the cytochrome P450 14alpha-demethylase (Erg11p, Cyp51p) to azole resistance in Candida albicans

28 October 2018

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Characterization and catalytic properties of the sterol 14alpha-demethylase from Mycobacterium tuberculosis

28 October 2018

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Generation of a complete, soluble, and catalytically active sterol 14 alpha-demethylase-reductase complex.

28 October 2018

1 reference

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Ligand interactions with cytochrome P-450. I. Binding of primary amines

28 October 2018

1 reference

12 December 2020

Metabolism of 32-hydroxy-24,25-dihydrolanosterol by purified cytochrome P-45014DM from yeast. Evidence for contribution of the cytochrome to whole process of lanosterol 14 alpha-demethylation

1 reference

12 December 2020

Interaction of microsomal cytochrome P-450 isolated from Aspergillus fumigatus with fluconazole and itraconazole

1 reference

12 December 2020

Isolation and Reconstitution of the Heme-Thiolate Protein Obtusifoliol 14α-Demethylase fromSorghum bicolor(L.) Moench

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12 December 2020

Evaluation of enzyme inhibitors in drug discovery. A guide for medicinal chemists and pharmacologists

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12 December 2020

Identifiers

DOI

10.1128/AAC.00316-10

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20660663%20AND%20SRC:MED&resulttype=core&format=json

8 February 2020

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20660663%20AND%20SRC:MED&resulttype=core&format=json

8 February 2020

PubMed publication ID

20660663

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20660663%20AND%20SRC:MED&resulttype=core&format=json