False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy (Q28249899)

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scientific article (publication date: April 2004)

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English	False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy	scientific article (publication date: April 2004)

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instance of

scholarly article

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review article

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Europe PubMed Central

title

False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy (English)

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author name string

A Borst

series ordinal

1

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A T A Box

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2

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A C Fluit

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3

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language of work or name

English

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publication date

April 2004

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published in

European Journal of Clinical Microbiology & Infectious Diseases

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volume

23

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issue

4

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page(s)

289-99

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cites work

Failure of ultra-violet irradiation and autoclaving to eliminate PCR contamination

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Detection of Candida spp. in blood cultures using nucleic acid sequence-based amplification (NASBA)

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Evidence of contamination in PCR laboratory disposables

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Single-step purification of a thermostable DNA polymerase expressed in Escherichia coli

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A past mutation at isoleucine 397 is now a common cause of moderate/mild haemophilia B.

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Termination sites of the in vitro nick-translation reaction on DNA that had photoreacted with psoralen

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Polymerase chain reaction decontamination: the wipe test

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Nucleic acid removal from Taq polymerase preparations using an aqueous/organic biphasic system

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Post-PCR sterilization: a method to control carryover contamination for the polymerase chain reaction

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Taq polymerase contains bacterial DNA of unknown origin.

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Reliability of PCR decontamination systems

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Removal of contaminating DNA from PCR reagents by ultrafiltration

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Primers determine the sensitivity of PCR-mediated hepatitis B virus DNA detection and pretreatment of PCR mixture with 8-methoxypsoralen eliminates false-positive results

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Nucleic acid sequence-based amplification

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Evacuated tubes and possible false-positive PCR results with blood samples

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More false-positive problems

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Post-PCR sterilization: development and application to an HIV-1 diagnostic assay

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Reliability of nucleic acid amplification for detection of Mycobacterium tuberculosis: an international collaborative quality control study among 30 laboratories

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Systemic candidiasis in cancer patients

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Eliminating PCR contamination: is UV irradiation the answer?

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Preventing false positives: quantitative evaluation of three protocols for inactivation of polymerase chain reaction amplification products

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Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli.

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Contaminations occurring in fungal PCR assays

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Qiagen DNA extraction kits for sample preparation for legionella PCR are not suitable for diagnostic purposes

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False-positive polymerase chain reaction results for human papillomavirus in lichen planus. Potential laboratory pitfalls of this procedure

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Use of UV irradiation to reduce false positivity in polymerase chain reaction.

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Branched DNA signal amplification

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Use of PCR primers containing a 3'-terminal ribose residue to prevent cross-contamination of amplified sequences

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Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions

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Extensive evaluation of a seronegative participant in an HIV-1 vaccine trial as a result of false-positive PCR

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Quantitation of cyclobutane pyrimidine dimer formation in double- and single-stranded DNA fragments of defined sequence

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Use of modified nucleotides and uracil-DNA glycosylase (UNG) for the control of contamination in the PCR-based amplification of RNA.

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Laboratory experience and guidelines for avoiding false positive polymerase chain reaction results.

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Environmental contamination leading to false-positive polymerase chain reaction for pertussis

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Identification and elimination of DNA sequences in Taq DNA polymerase.

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Frequent contamination of Taq polymerase with DNA

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The ligation amplification reaction (LAR)--amplification of specific DNA sequences using sequential rounds of template-dependent ligation

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Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion.

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The effects of covalent additions of a psoralen on transcription by E. coli RNA polymerase

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Decontaminating the polymerase chain reaction

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Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format

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Detection of DNA contamination in Taq polymerase

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UV and clean air result in contamination-free PCR

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GEMHEP multicenter quality control study of PCR detection of GB virus C/hepatitis G virus RNA in serum

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Rapid identification of bacteria by PCR-single-strand conformation polymorphism

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Protocol for ultraviolet irradiation of surfaces to reduce PCR contamination

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Reliability of methods for hepatitis B virus DNA detection

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Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens.

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Quality control for DNA contamination in laboratories using PCR-based class II HLA typing methods

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Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia

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False-positive results and the polymerase chain reaction

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Contamination and sensitivity issues with a real-time universal 16S rRNA PCR

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Exclusion of false-positive PCR viral diagnosis by single-strand conformation polymorphism

1 reference

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Sites of termination of in vitro DNA synthesis on psoralen phototreated single-stranded templates

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Use of psoralen as extinguisher of contaminated DNA in PCR.

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False‐Positive Results of PCR Testing for Lyme Disease

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A novel method employing UNG to avoid carry-over contamination in RNA-PCR.

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DNase I treatment of Taq DNA polymerase for complete PCR decontamination

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A systematic and quantitative analysis of PCR template contamination

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Strand displacement amplification--an isothermal, in vitro DNA amplification technique

1 reference

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Shedding light on PCR contamination

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UV Irradiation of Polystyrene Pipets Releases PCR Inhibitors

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PCR: how to kill unwanted DNA

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Dealing with contamination: enzymatic control of carryover contamination in PCR

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Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology

1 reference

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reference URL

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Application of hydroxylamine hydrochloride for post-PCR sterilization

1 reference

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reference URL

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inferred from PubMed ID database lookup

Polymerase chain reaction: trenches to benches

1 reference

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PubMed

reference URL

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retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Reliability of polymerase chain reaction for detection of hepatitis C virus

1 reference

stated in

PubMed

reference URL

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inferred from PubMed ID database lookup

False-Positive Polymerase Chain Reaction Results in the Diagnosis of Herpes Simplex Encephalitis

1 reference

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reference URL

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Improving PCR efficiency

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reference URL

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based on heuristic

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A statistical method for the detection of false positives and false negatives in microtitre format PCR assays

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15015033

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based on heuristic

inferred from PubMed ID database lookup

Avoiding false positives with PCR

1 reference

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reference URL

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based on heuristic

inferred from PubMed ID database lookup

Effect of nucleotide analogs on the cleavage of DNA by the restriction enzymes AluI, DdeI, HinfI, RsaI, and TaqI.

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15015033

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Use of gamma irradiation to eliminate DNA contamination for PCR.

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15015033

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Parameters affecting susceptibility of PCR contamination to UV inactivation

1 reference

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PubMed

reference URL

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12 December 2020

based on heuristic

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False-positive diagnosis of tuberculosis with PCR

1 reference

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reference URL

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based on heuristic

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Death from inappropriate therapy for Lyme disease

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reference URL

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Utilizing uracil DNA glycosylase to control carryover contamination in PCR: characterization of residual UDG activity following thermal cycling

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15015033

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12 December 2020

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The False-Positive Polymerase Chain Reaction and the Ostrich

1 reference

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retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Avoidance of false positives

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15015033

retrieved

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based on heuristic

inferred from PubMed ID database lookup

Amplification of residual DNA sequences in sterile bronchoscopes leading to false-positive PCR results.

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15015033

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Characterization of contaminating DNA in Taq polymerase which occurs during amplification with a primer set for Legionella 5S ribosomal RNA.

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15015033

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Source of contamination in polymerase chain reaction assay

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15015033

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identification of contaminating fungal DNA sequences in Zymolyase.

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15015033

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

More light on PCR contamination

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15015033

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Removal of DNA contamination in polymerase chain reaction reagents by ultraviolet irradiation

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15015033

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

DOI

10.1007/S10096-004-1100-1

1 reference

stated in

Consolidated OpenCitations Corpus – April 2017

OpenCitations bibliographic resource ID

971255

Dimensions Publication ID

1016532613

0 references

OpenCitations bibliographic resource ID

971255

1 reference

stated in

Consolidated OpenCitations Corpus – April 2017

OpenCitations bibliographic resource ID

971255

PubMed publication ID

15015033

1 reference

stated in

Consolidated OpenCitations Corpus – April 2017

OpenCitations bibliographic resource ID

971255

 

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