Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis (Q24296676)

6 July 2017

title

Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis (English)

1 reference

6 July 2017

0 references

Nikolaus Grigorieff

Nikolaus

Grigorieff

1 reference

6 July 2017

Melissa J Moore

Melissa J

Moore

1 reference

6 July 2017

Melissa S Jurica

Melissa S

Jurica

1 reference

6 July 2017

Lawrence J Licklider

series ordinal

author given names

Lawrence J

author last names

Licklider

1 reference

6 July 2017

Steven R Gygi

series ordinal

author given names

Steven R

author last names

Gygi

1 reference

6 July 2017

language of work or name

English

0 references

publication date

1 April 2002

1 reference

6 July 2017

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6 July 2017

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6 July 2017

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6 July 2017

page(s)

426-439

1 reference

Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex

6 July 2017

cites work

1 reference

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The 65 and 110 kDa SR-related proteins of the U4/U6.U5 tri-snRNP are essential for the assembly of mature spliceosomes

19 March 2017

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The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions

19 March 2017

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Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae

19 March 2017

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Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry

19 March 2017

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Functional association of U2 snRNP with the ATP-independent spliceosomal complex E

19 March 2017

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Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing

19 March 2017

1 reference

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The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes

19 March 2017

1 reference

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Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing

19 March 2017

1 reference

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Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex

19 March 2017

1 reference

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Crystal structure of the spliceosomal U2B"-U2A' protein complex bound to a fragment of U2 small nuclear RNA

19 March 2017

1 reference

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A coactivator of pre-mRNA splicing

19 March 2017

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Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing

19 March 2017

1 reference

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An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2

19 March 2017

1 reference

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A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Identification of hnRNP P2 as TLS/FUS using electrospray mass spectrometry.

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Structure of the 30S ribosomal subunit

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP.

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Yeast ortholog of the Drosophila crooked neck protein promotes spliceosome assembly through stable U4/U6.U5 snRNP addition

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Mechanical devices of the spliceosome: motors, clocks, springs, and things

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

A new generation of the IMAGIC image processing system

19 March 2017

1 reference

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Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000.

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

RNA polymerase II and the integration of nuclear events

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Proteomics: the move to mixtures

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

The protein Mago provides a link between splicing and mRNA localization.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

The Y14 protein communicates to the cytoplasm the position of exon-exon junctions

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Evidence for a linear search in bimolecular 3' splice site AG selection

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Better conditions for mammalian in vitro splicing provided by acetate and glutamate as potassium counterions

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Spliceosomal U snRNP core assembly: Sm proteins assemble onto an Sm site RNA nonanucleotide in a specific and thermodynamically stable manner

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Structural studies of the translational apparatus

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Prp43: An RNA helicase-like factor involved in spliceosome disassembly

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

The second catalytic step of pre-mRNA splicing

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

PRP19: a novel spliceosomal component

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Identification of a functional mammalian spliceosome containing unspliced pre-mRNA

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Differential binding of heterogeneous nuclear ribonucleoproteins to mRNA precursors prior to spliceosome assembly in vitro

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

The three-dimensional structure of the ribosome and its components

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Mutations within the yeast U4/U6 snRNP protein Prp4 affect a late stage of spliceosome assembly

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Purification and visualization of native spliceosomes

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Protein components specifically associated with prespliceosome and spliceosome complexes

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Bimolecular exon ligation by the human spliceosome bypasses early 3' splice site AG recognition and requires NTP hydrolysis

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Mutants of the bacteriophage MS2 coat protein that alter its cooperative binding to RNA.

29 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1370266

Bimolecular exon ligation by the human spliceosome

29 November 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/11991638

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

A multicomponent complex is involved in the splicing of messenger RNA precursors

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/11991638

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1017/S1355838202021088

1 reference

release_mxpaytbtfnbwfkss722tk2y4um

6 July 2017

Fatcat ID

0 references

OpenCitations bibliographic resource ID

4420716

1 reference

Consolidated OpenCitations Corpus – April 2017

OpenCitations bibliographic resource ID

1 reference

6 July 2017

1 reference