Scientific Interest

DNA damage and Repair, Chemistry and Biochemistry of DNA & RNA
Radiation Biology, Photobiology, Oxidative stress, Environmental Toxicology, Nanotoxicology, Radiosensitization
Mechanisms of DNA damage formation, Detection of cellular DNA damages, epigenetic DNA modifications

Background

Licence in Biochemistry (1989, Montpellier, France)
Master in Organic Chemistry (1989, Grenoble, France)
Ph.D. in Organic Chemistry (1992, Grenoble, France)
Habilitation (HRD) (1999, Grenoble, France)

Experience

1989-1992: Ph. D. student, laboratory "Lésions des Acides Nucléiques", CEA-Grenoble (Supervisor Dr J. Cadet).
1993-1995: Post-doctoral fellow, Laboratory "Metabolism of Xenobiotics", Quality and Safety Assurance Department, Nestlé Research Centre, Switzerland.
1995-2016: Research Scientist, CEA-Grenoble, Laboratory "Lésions des Acides Nucléiques"
2012-2016: Head of the laboratory "Lésions des Acides Nucléiques"
Since 2017: Research Scientist laboratory "Chimie Interface Biologie, pour l'Environnement, la Santé et la Toxicology"
Since 2011: Research Director at CEA.

Competences

Chemical synthesis (modified DNA lesions, including isotopically labeled compounds)
Analytical chemistry (HPLC, GC, Immunoaffinity chromatography,...) with special interest in HPLC coupled through electrospray ionization to tandem mass spectrometry using isotopically labeled internal standards.
Spectroscopy: One and two dimensional NMR (¹H, ¹³C, ¹⁵N), mass spectrometry (ESI-ions trap, Maldi-TOF) , FT-IR,...
Biochemistry: Endo- and Exo-nucleases, DNA repair enzymes, monoclonal antibodies, single cell gel electrophoresis (comet assay), cell culture.

Award

"Young investigator award" delivered by the International Society for Free Radical Research, Barcelona, Spain 1996

Scientific activities

Involved in the evaluation processes of several scientific journals: J. Am. Chem. Soc, Nat. Methods., Chem. Res. Toxicol., Photochem. Photobiol., Free Rad Biol. Med., Radiat. Res., Int. J. Radiat. Biol., New J. Chem., Photochem. Photobiol. Sci., Rapid Commun. Mass. Spec., Cancer Sci., Biochemistry, Anal. Chem., J Phys. Chem., Mutat. Res.…
Evaluation of numerous international grant proposals, HCERES Expert,…

Co-authored of more than 180 publications (H-index 64) and 30 book chapters.

List of principal recent publications

DNA Damage formation & Repair
Taverna Porro, M. L., Saint-Pierre, C., Gasparutto, D., and Ravanat, J. L. (2020) Solid-phase synthesis of branched oligonucleotides containing a biologically relevant dCyd341 interstrand crosslink DNA lesion. Org Biomol Chem 18, 1892-1899.

Lebraud, E., Pinna, G., Siberchicot, C., Depagne, J., Busso, D., Fantini, D., Irbah, L., Robeska, E., Kratassiouk, G., Ravanat, J. L., Epe, B., Radicella, J. P., and Campalans, A. (2020) Chromatin recruitment of OGG1 requires cohesin and mediator and is essential for efficient 8-oxoG removal. Nucleic Acids Res 48, 9082-8097.

Charazac, A., Fayyad, N., Beal, D., Bourgoin-Voillard, S., Seve, M., Sauvaigo, S., Lamartine, J., Soularue, P., Moratille, S., Martin, M. T., Ravanat, J. L., Douki, T., and Rachidi, W. (2020) Impairment of Base Excision Repair in Dermal Fibroblasts Isolated From Nevoid Basal Cell Carcinoma Patients. Front Oncol 10, 1551.

Bignon, E., Chan, C. H., Morell, C., Monari, A., Ravanat, J. L., and Dumont, E. (2017) Molecular Dynamics Insights into Polyamine-DNA Binding Modes: Implications for Cross-Link Selectivity. Chem. Eur. J 23, 12845.

Gomez Mendoza, M., Banyasz, A., Douki, T., Markovitsi, D., and Ravanat, J. L. (2016) Direct Oxidative Damage of Naked DNA Generated upon Absorption of UV Radiation by Nucleobases. J. Phys. Chem. Lett. 7, 3945-3948.

Dumont, E., Grüber, R., Bignon, E., Morell, C., Randa, J., Ravanat, J.-L., and Tunon, I. (2016) Singlet oxyen attack on guanine: reactivity and structural signature within the B-DNA helix. Chem. Eur. J. 22, 12358_12362.

Silerme, S., Bobyk, L., Taverna-Porro, M., Cuier, C., Saint-Pierre, C. and Ravanat, J.L. (2014) DNA-polyamine crosslinks generated upon one electron oxidation of DNA. Chem Res Toxicol, 27, 1011-1018.

Radiosensitization
Bulin, A.-L., Broekgaarden, M., Chaput, F., Baisamy, V., Garrevoet, J., Busser, B., Brueckner, D., Youssef, A., Ravanat, J.-L., Dujardin, C., Motto-Ros, V., Lerouge, F., Bohic, S., Sancey, L., and Elleaume, H. (2020) Radiation Dose-Enhancement Is a Potent Radiotherapeutic Effect of Rare-Earth Composite Nanoscintillators in Preclinical Models of Glioblastoma. Advanced Science 7, 2001675.

Delorme, R., Taupin, F., Flaender, M., Ravanat, J. L., Champion, C., Agelou, M., and Elleaume, H. (2017) Comparison of Gadolinium Nanoparticles and Molecular Contrast Agents for Radiation Therapy-Enhancement. Med Phys 44, 5949-5960.

Bayart, E., Pouzoulet, F., Calmels, L., Dadoun, J., Allot, F., Plagnard, J., Ravanat, J. L., Bridier, A., Denoziere, M., Bourhis, J., and Deutsch, E. (2017) Enhancement of IUdR Radiosensitization by Low-Energy Photons Results from Increased and Persistent DNA Damage. PLoS One 12, e0168395.

Bobyk, L., Edouard, M., Deman, P., Vautrin, M., Pernet-Gallay, K., Delaroche, J., Adam, J. F., Esteve, F., Ravanat, J. L., and Elleaume, H. (2013) Photoactivation of gold nanoparticles for glioma treatment. Nanomedicine 9, 1089-1097.

Bobyk, L., Edouard, M., Deman, P., Rousseau, J., Adam, J. F., Ravanat, J. L., Esteve, F., Balosso, J., Barth, R. F., and Elleaume, H. (2012) Intracerebral delivery of Carboplatin in combination with either 6 MV Photons or monoenergetic synchrotron X-rays are equally efficacious for treatment of the F98 rat glioma. J. Exp. Clin. Cancer Res. 31, 78.

Review articles
Pouget, J. P., Georgakilas, A. G., and Ravanat, J. L. (2018) Targeted and Off-Target (Bystander and Abscopal) Effects of Radiation Therapy: Redox Mechanisms and Risk/Benefit Analysis. Antioxid Redox Signal 29, 1447-1487.

Hall, J., Jeggo, P. A., West, C., Gomolka, M., Quintens, R., Badie, C., Laurent, O., Aerts, A., Anastasov, N., Azimzadeh, O., Azizova, T., Baatout, S., Baselet, B., Benotmane, M. A., Blanchardon, E., Guéguen, Y., Haghdoost, S., Harms-Ringhdahl, M., Hess, J., Kreuzer, M., Laurier, D., Macaeva, E., Manning, G., Pernot, E., Ravanat, J.-L., Sabatier, L., Tack, K., Tapio, S., Zitzelsberger, H., and Cardis, E. (2017) Ionizing radiation biomarkers in epidemiological studies – An update. Mutation Research/Reviews in Mutation Research 771, 59-84.

Carriere, M., Sauvaigo, S., Douki, T., and Ravanat, J. L. (2017) Impact of nanoparticles on DNA repair processes: current knowledge and working hypotheses. Mutagenesis 3, 303-213.

Ravanat, J. L., and Douki, T. (2016) UV and ionizing radiations induced DNA damage, differences and similarities. J. Rad. Phys. Chem. 128, 92-102.

Ferreri, C., Golding, B. T., Jahn, U., and Ravanat, J. L. (2016) COST ACTION CM1201 "Biomimetic Radical Chemistry": free radical chemistry successfully meets many disciplines. Free Radic Res 50S1, 1-40.

Cadet, J., Douki, T., and Ravanat, J. L. (2015) Oxidatively generated damage to cellular DNA by UVB and UVA radiation. Photochem Photobiol 91, 140-155.

Complete list of publications

Updated January 2021 - pdf
Link to publications through PubMed.