[go: up one dir, main page]

Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Research Briefing
  • Published:

Dedifferentiating myoblasts back into a satellite cell state in vitro

Satellite cells, the stem cells of skeletal muscle, are responsible for muscle development and regeneration. Although low in abundance, satellite cells can be isolated from muscle but cannot be propagated successfully in culture in numbers needed for therapeutic use. We developed a method to generate cells with satellite cell characteristics from skeletal muscle organoid cultures.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: IdSCs regenerate skeletal muscle that produces contractile force in vivo.

References

  1. Mauro, A. Satellite cell of skeletal muscle fibers. J. Biophys. Biochem. Cytol. 9, 493–495 (1961). This paper identified the satellite cell and postulated its role as a stem cell that is responsible for skeletal muscle regeneration.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Montarras, D. & Buckingham, M. Direct isolation of satellite cells for skeletal muscle regeneration. Science 309, 2064–2067 (2005). This paper stands as a great example of how satellite cells lose their stem cell function following traditional culture in vitro.

    Article  CAS  PubMed  Google Scholar 

  3. Judson, R. N. & Rossi, F. M. V. Towards stem cell therapies for skeletal muscle repair. npj Regen Med. 5, 10 (2020). This review provides a summary of recent progress in the field of skeletal muscle cell therapy.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Seale, P. & Rudnicki, M. A. Pax7 is required for the specification of myogenic satellite cells. Cell. 102, 777–786 (2000). This paper identified PAX7 as a key transcription factor that is present in adult satellite cells.

    Article  CAS  PubMed  Google Scholar 

Download references

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This is a summary of: Price, F. D. et al. Organoid culture promotes dedifferentiation of mouse myoblasts into stem cells capable of complete muscle regeneration. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02344-7 (2024).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dedifferentiating myoblasts back into a satellite cell state in vitro. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02345-6

Download citation

  • Published:

  • DOI: https://doi.org/10.1038/s41587-024-02345-6

Search

Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research