Extended Data Fig. 4: Sleep phenotypes of Ppp1r9b KO mice.
From: Postsynaptic competition between calcineurin and PKA regulates mammalian sleep–wake cycles
(a, b) The genotyping of Ppp1r9b KO mice. qPCR was performed with primer pairs listed in SI Table 1 for the three target sites in a gene. When 0.5% criteria were met in either set, the mouse was considered a KO mouse. Each number represents each mouse used for the genotyping, and magenta color indicates a KO-determined animal. (c) 24-hour sleep profiles of Ppp1r9b KO mice demonstrated in Fig. 3b.(d, e) Sleep/wake parameters of Ppp1r9b KO (set 2) mice, averaging over six days (d) and sleep profiles (e). KO mice were compared with 8-week-old WT C57BL/6N male mice. The black broken line, and the shaded area represent the mean and one s.d. range, respectively, of the WT mice. (f) Sleep phenotypes of first and second half day of Ppp1r9b KO mice and WT C57BL/6N mice indicated in Fig. 3c–e. (g) NREM power density in delta domain (1–4 Hz) and EEG power spectra of Ppp1r9b KO mice (n = 8) and WT C57BL/6N mice (n = 8) depicted in Fig. 3c–e. (h) Differences in transition probabilities (between wakefulness (W), NREM sleep (N), and REM sleep (R)) between WT C57BL/6N mice and Ppp1r9b KO mice. Magenta lines and broken blue lines indicate significantly (p < 0.05) higher and lower values for Ppp1r9b KO mice, respectively. The number of mice used in the analysis has been depicted as (n). Data are mean ± s.d., while shaded areas in the line plots represent mean ± s.e.m. The sample sizes for each experiment, the statistical methods used, and the statistical significance are summarized in SI Table 2. *p < 0.05, **p < 0.01, ***p < 0.001. KO: knock out; WT: wild-type; ZT: zeitgeber time.