Extended Data Fig. 5: eIF1 preferentially associates with the ribosome during mitosis.
From: Nuclear release of eIF1 restricts start-codon selection during mitosis
a, Heatmap showing successful fractionation of ribosomes and quantitative nature of mass spectrometry from interphase cells. Top cluster represents 40S subunits while bottom represents 60S subunits. Plotted is the average Z-score of 2 biological replicates. b, Biological replicates from interphase (left) and mitotic (right) 40S fraction TMT mass spectrometry experiments. c, Rank-ordered relative fold-change in the abundance of translation initiation factors and ribosomal proteins in free, 60S, and 80S ribosome fractions. Each point represents the average fold change between 2 biological replicates and the shading is the standard error of the mean. Interestingly eIF5B and eIF5A are also regulators of start-codon selection, with eIF5B acting directly and eIF5A indirectly40,41,54,55. d, Western blot analysis of eIF1 and eIF5 protein expression in interphase, cycling mitotic, and mitotically arrested HeLa cells.