The authors provide an update to their 2006 report on the successful large-scale captive breeding of the 13-lined ground squirrel (TLGS; Ictidomys tridecemlineatus) in their colony, now a single-source supplier of purpose-bred TLGSs for several research institutions. With funding from the National Institutes of Health, the authors have expanded their capacity for breeding pairs and built a dedicated hibernaculum to house torpid animals in the facility. The authors report new information on housing, diet, environmental enrichment, breeding, behavior and health. They also report the findings of several in-house studies on the health, behavior and well-being of their TLGSs.