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Pulmonary surfactant obtained from starved rats enhances phagocytosis of alveolar macrophages

Cell Mol Biol. 1991;37(4):475-80.

Abstract

Phagocytic activity of alveolar macrophages (AM) was enhanced by pulmonary surfactant obtained from bronchoalveolar lavage fluid of rats starved for 2 days, as compared to fed. The enhanced activity of phagocytosis was dependent on the dose of surfactant. The prepared surfactant showed a different protein to phospholipid ratio of 0.108 in fed and 0.234 in 2 days starved, because of an increased ratio of protein in surfactant from 2 days starved rats. F(ab')2 anti-surfactant protein inhibited the enhanced AM phagocytosis by surfactant. These results suggested that the enhancement of AM phagocytosis in 2 days starved rats was on account of an increase of protein in their surfactant compared to fed.

MeSH terms

  • Animals
  • Bronchoalveolar Lavage Fluid / chemistry
  • Immunoglobulin Fab Fragments / immunology
  • Macrophages, Alveolar / drug effects
  • Macrophages, Alveolar / immunology*
  • Male
  • Phagocytosis / drug effects*
  • Phospholipids / analysis
  • Proteins / analysis
  • Pulmonary Surfactants / chemistry
  • Pulmonary Surfactants / immunology
  • Pulmonary Surfactants / pharmacology*
  • Rats
  • Rats, Inbred F344
  • Starvation / immunology*

Substances

  • Immunoglobulin Fab Fragments
  • Phospholipids
  • Proteins
  • Pulmonary Surfactants