TABLE 1.
Affinity of specific oxPAPC components for D-4F, L-4F, and apoA-I
|
KD |
|||
|---|---|---|---|
| Lipids | D-4F | L-4F | apoA-I |
| nM | |||
| PAPC | 118,576 ± 36,843 | 192,821 ± 56,505 | 99,871 ± 14,114 |
| PGPC | 6.3 ± 4.2 | 7.2 ± 4.5 | 4,206 ± 4,228 |
| POVPC | 2.4 ± 2.0 | 3.6 ± 2.6 | 30,852 ± 28,314 |
| PEIPC | 0.06 ± 0.05 | 0.01 ± 0.01 | 50,720 ± 5,721 |
| KOdiA-PC | 0.58 ± 0.0 | 1.58 ± 3.5 | 20,960 ± 11,680 |
apoA-I, apolipoprotein A-I; PAPC, 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine; PGPC, 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphorylcholine; POVPC, 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphorylcholine; PEIPC, 1-palmitoyl-2-(5,6-epoxyisoprostane E2)-sn-glycero-3-phosphorylcholine; KOdiA-PC, 1-palmitoyl-2-(5-keto-6-octene-dioyl)-sn-glycero-phosphatidylcholine. Binding was determined by surface plasmon resonance (SPR), as described in Materials and Methods. The values shown are the mean ± SEM from five separate experiments.