Figure 1.
Expression of VEGF transcripts in HMC-1 cells. Semiquantitative RT-PCR analysis of mRNA from unstimulated HMC-1 cells (lane 3) and cells stimulated for 24 h with PMA/A23187 (lane 4). (A) The primers chosen for this amplification allowed detection of all VEGF isoforms. (B) A VEGF206-specific primer pair was chosen (Figure 2A), and the product amplified was cut from the gel and identified by direct sequencing (Figure 2B). lane 1, size marker; lane 2, negative control (sample without cDNA). The positions of the size markers are on the left side and the sizes of the amplified products are indicated on the right side.