Abstract
Some characteristics of inhibition of cell migration induced in tissue culture by the addition of specific antigen were studied. The following characteristics were found to be shared by this type of cellular hypersensitivity and delayed cutaneous sensitivity: 1. Specificity for the carrier moiety of haptene protein conjugates. The picryl protein conjugate used to sensitize guinea pigs inhibited migration of monocytic cells from these animals. Other picrylated proteins produced little inhibition. 2. Enhancement by mycobacterial adjuvants. Incorporation of tubercle bacilli with picrylated proteins in adjuvant-antigen emulsions stimulated the development of this cellular hypersensitivity to antigen. 3. Independence of circulating antibody. In contrast to cellular hypersensitivity, serum antibody (a) reacted with any of a number of picrylated proteins, (b) developed well in the absence of mycobacterial adjuvant, and (c) persisted in unchanged titer for 5 weeks in animals sensitized with saline solutions of antigen. During this time cellular hypersensitivity decreased remarkably. The in vitro system described provides a direct method to measure cell-antigen interaction and permits study of an aspect of the immune response not mediated by humoral antibody. The relation of cellular hypersensitivity to antibody formation and delayed hypersensitivity is discussed.
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Selected References
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- BENACERRAF B., LEVINE B. B. Immunological specificity of delayed and immediate hypersensitivity reactions. J Exp Med. 1962 May 1;115:1023–1036. doi: 10.1084/jem.115.5.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BENACERRAF B., OJEDA A., MAURER P. H. STUDIES ON ARTIFICIAL ANTIGENS. II. THE ANTIGENICITY IN GUINEA PIGS OF ARSANILIC ACID CONJUGATES OF COPOLYMERS OF D- OR L-ALPHA-AMINO ACIDS. J Exp Med. 1963 Dec 1;118:945–952. doi: 10.1084/jem.118.6.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CARPENTER R. R. IN VITRO STUDIES OF CELLULAR HYPERSENSITIVITY. I. SPECIFIC INHIBITION OF MIGRATION OF CELLS FROM ADJUVANT-IMMUNIZED ANIMALS BY PURIFIED PROTEIN DERIVATIVE AND OTHER PROTEIN ANTIGENS. J Immunol. 1963 Dec;91:803–818. [PubMed] [Google Scholar]
- GEORGE M., VAUGHAN J. H. In vitro cell migration as a model for delayed hypersensitivity. Proc Soc Exp Biol Med. 1962 Nov;111:514–521. doi: 10.3181/00379727-111-27841. [DOI] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : III. THE REACTION BETWEEN CRYSTALLINE EGG ALBUMIN AND ITS HOMOLOGOUS ANTIBODY. J Exp Med. 1935 Oct 31;62(5):697–720. doi: 10.1084/jem.62.5.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KANTOR F. S., OJEDA A., BENCARERRAF B. Studies on artifical antigens. I. Antigenicity of DNP-polylysine and DNP copolymer of lysine and glutamic acid in guinea pigs. J Exp Med. 1963 Jan 1;117:55–69. doi: 10.1084/jem.117.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- OVARY Z., BENACERRAF B., BLOCH K. J. Properties of guinea pig 7S antibodies. II. Identification of antibodies involved in passive cutaneous and systemic anaphylaxis. J Exp Med. 1963 Jun 1;117:951–964. doi: 10.1084/jem.117.6.951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RAFFEL S., ARNAUD L. E. The role of the wax of the tubercle bacillus in establishing delayed hypersensitivity, hypersensitivity to a protein antigen, egg albumin. J Exp Med. 1949 Jul;90(1):53–72. doi: 10.1084/jem.90.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALVIN S. B., SMITH R. F. The specificity of allergic reactions. I. Delayed versus Arthus hypersensitivity. J Exp Med. 1960 Apr 1;111:465–483. doi: 10.1084/jem.111.4.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TALMAGE D. W., BAKER H. R., AKESON W. The separation and analysis of labelled antibodies. J Infect Dis. 1954 Mar-Apr;94(2):199–212. doi: 10.1093/infdis/94.2.199. [DOI] [PubMed] [Google Scholar]
- TALMAGE D. W., MAURER P. H. I131-Labelled antigen precipitation as a measure of quantity and quality of antibody. J Infect Dis. 1953 May-Jun;92(3):288–300. doi: 10.1093/infdis/92.3.288. [DOI] [PubMed] [Google Scholar]