Abstract
Healing skin wounds were studied in a series of parabiotic rats. The femurs of one parabiont of each pair were shielded whilst both animals were given 800 r from a Co60 source. The animals were wounded 3 days after irradiation. Each animal with partially shielded marrow was then given tritiated thymidine intraperitoneally daily while the cross-circulation was arrested by clamping. After the thymidine-3H had cleared the blood, the clamp was released. Animals were sacrificed, and wounds were prepared for radioautography 1, 2, and 6 days after wounding. In the wounds of the shielded animals thymidine-3H was observed in epidermis, endothelium, leukocytes, fibroblasts, and mast cells. Only neutrophilic leukocytes, monocytes, and lymphocytes were labeled, as determined by light and electron microscope radioautography, in the wounds of each nonshielded parabiont. None of the many fibroblasts present were found to contain label in the wounds of the nonshielded parabionts through the 6 day period. These observations provide further evidence that wound fibroblasts do not arise from hematogenous precursors and, therefore, must arise from adjacent connective tissue cells.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dodd R. M., Sigel B., Dunn M. R. Localization of new cell formation in tendon healing by tritiated thymidine autoradiography. Surg Gynecol Obstet. 1966 Apr;122(4):805–806. [PubMed] [Google Scholar]
- FARNES P., BARKER B. E. Cytochemical studies of human bone marrow fibroblast-like cells. I. Alkaline phosphatase. Exp Cell Res. 1963 Jan;29:278–288. doi: 10.1016/0014-4827(63)90383-5. [DOI] [PubMed] [Google Scholar]
- GIESEKING R. SUBMIKROSKOPISCHE STRUKTURUNTERSCHIEDE ZWISCHEN HISTIOZYTEN UND FIBROBLASTEN. Beitr Pathol Anat. 1963 Jul;128:259–282. [PubMed] [Google Scholar]
- GRILLO H. C. DERIVATION OF FIBROBLASTS IN THE HEALING WOUND. Arch Surg. 1964 Feb;88:218–224. doi: 10.1001/archsurg.1964.01310200056013. [DOI] [PubMed] [Google Scholar]
- GRILLO H. C. Origin of fibroblasts in wound healing. An autoradiographic study of inhibition of cellular proliferation by local x-irradiation. Ann Surg. 1963 Mar;157:453–467. doi: 10.1097/00000658-196303000-00018. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRILLO H. C., POTSAID M. S. Studies in wound healing. IV. Retardation of contraction by localx-irradiation, and observations relating to the origin of fibroblasts in repair. Ann Surg. 1961 Nov;154:741–750. [PMC free article] [PubMed] [Google Scholar]
- HADFIELD G. THE TISSUE OF ORIGIN OF THE FIBROBLASTS OF GRANULATION TISSUE. Br J Surg. 1963 Sep;50:870–881. doi: 10.1002/bjs.18005022627. [DOI] [PubMed] [Google Scholar]
- MACDONALD R. A. Origin of fibroblasts in experimental healing wounds: autoradiographic studies using tritiated thymidine. Surgery. 1959 Aug;46(2):376–382. [PubMed] [Google Scholar]
- PETRAKIS N. L., DAVIS M., LUCIA S. P. The in vivo differentiation of human leukocytes into histiocytes, fibroblasts and fat cells in subcutaneous diffusion chambers. Blood. 1961 Jan;17:109–118. [PubMed] [Google Scholar]
- PETRAKIS N. L. In vivo cultivation of leukocytes in diffusion chambers: requirement of ascorbic acid for differentiation of mononuclear leukocytes to fibroblasts. Blood. 1961 Sep;18:310–316. [PubMed] [Google Scholar]
- Rangan S. R. Origin of the fibroblastic growths in chicken buffy coat macrophage cultures. Exp Cell Res. 1967 Jun;46(3):477–487. doi: 10.1016/0014-4827(67)90374-6. [DOI] [PubMed] [Google Scholar]
- Ross R., Lillywhite J. W. The fate of buffy coat cells grown in subcutaneously implanted diffusion chambers. A light and electron microscopic study. Lab Invest. 1965 Sep;14(9):1568–1585. [PubMed] [Google Scholar]
- Ross R. The fibroblast and wound repair. Biol Rev Camb Philos Soc. 1968 Feb;43(1):51–96. doi: 10.1111/j.1469-185x.1968.tb01109.x. [DOI] [PubMed] [Google Scholar]
- SHELTON E., RICE M. E. Growth of normal peritoneal cells in diffusion chambers: a study in cell modulation. Am J Anat. 1959 Nov;105:281–341. doi: 10.1002/aja.1001050302. [DOI] [PubMed] [Google Scholar]
- WEIMAR V. L. The sources of fibroblasts in corneal wound repair; a quantitative analysis. AMA Arch Ophthalmol. 1958 Jul;60(1):93–109. doi: 10.1001/archopht.1958.00940080107014. [DOI] [PubMed] [Google Scholar]