Figure 1.
Targeting of the mouse endothelial lipase gene. (a) The wild-type locus of mouse LIPG (top), the targeting construct (middle), and the targeted locus (bottom). Exon 1 was replaced with the neomycin phosphotransferase gene (neo), and recombination was detected by Southern blot analysis using the probes indicated. X, XbaI; B, BamHI; K, KpnI; Bg, BglII; H, HindIII; E, EcoRI; Xh, XhoI. (b) Both probes gave a 20-kb wild-type band on Southern blotting, whereas the targeted recombinants showed a 6.8-kb band with the 5′probe and a 13.2-kb band with the 3′probe. Targeted stem cells were characterized by the presence of both DNA fragments. (c) Northern blot analysis showed LIPG expression in mouse tissues. A full-length mouse LIPG cDNA was used as a probe. Cyph, cyclophilin.