[go: up one dir, main page]
Skip to main content
. 2021 Feb 15;52:21. doi: 10.1186/s13567-020-00888-5

Table 1.

Major 3D skin models in humans and mice

Type of models Species Specificities References
Skin explants cultivated ex vivo (from biopsies) Mouse Skin explants cultured on chick chorioallantoic membrane (CAM) [22]
Reconstructed human epidermis: on an acellular de-epidermized dermis (aDED) support Human Primary keratinocytes cultured onto an aDED to reconstruct an epidermis. The keratinocytes being differentiated through ALI [81, 83]
Reconstructed mouse epidermis: on an acellular de-epidermized dermis (aDED) support Mouse [84]
Reconstructed human epidermis: on plastic filter support Human Keratinocytes cultured at ALI on an inert surface (e.g. polycarbonate insert filter) [91, 92]
Reconstructed mouse epidermis: on plastic filter support Mouse [94]
Reconstructed human epidermis: on a dermal substitute made of biomaterials Human Seeding of keratinocytes on a dermal substitute made of biomaterials (collagen, hyaluronic acid, alginate…). A multilayered epidermis is obtained following the differentiation of keratinocytes at ALI [89, 95]
Full thickness skin: on dermal substitute made of biomaterials and populated with fibroblasts Human Seeding of keratinocytes on a living dermal substitute (collagen mixed with fibroblasts). A multilayered skin equivalent is obtained following the differentiation of keratinocytes at the ALI [99–101]
Mouse Mouse skin epidermis model. Mouse keratinocytes cultured at ALI on a living dermal substitute (type-I collagen with murine dermal fibroblasts) [102]

Full thickness skin:

DED populated with fibroblasts

 Human Keratinocytes seeded on a DED populated with fibroblasts and cultured at ALI [97]
Full thickness skin: combining a DED populated with fibroblasts Human Keratinocytes seeded on a DED populated with fibroblasts and cultured at ALI [98]
Complex Full thickness skin: pigmented models Human Skin equivalent additionated with melanocytes [121]
Complex Full thickness skin: skins diseases related models Human Psoriasis or melanoma models [123, 127]
Immunocompetent 3D skin equivalent Human 3D skin model comprising of: dendritic cells (incl. Langerhans cells) or T-cells [120, 123–125]
Full thickness skin: reconstituted from immortalized cells Human 3D skin culture techniques using immortalized keratinocytes and fibroblasts to produce a multilayered human skin equivalent [109, 110]
Full thickness skin: reconstituted from iPSCs-derived cells Human Multilayered epidermal structure reconstituted from human keratinocytes and fibroblasts cells derived from iPSCs [113]
Full thickness hair-bearing skin: organoid system Human Multilayered epidermal structure exhibiting hairs reconstituted thanks to an organoid system [117]
3D Skin obtained by bioprinting Human Automated process used to overlay sheets of cells to obtain a complex skin architecture [129]
Skin-on-chip systems Human Technology combining reconstructed skin tissues with microsystems [132]