Extended Data Figure 3. qPCR confirmation of the RNAseq data.
(a) qPCR determination of the modulation of specific SLCs during efferocytosis. Indicated SLC genes were tested for mRNA expression levels during engulfment assays performed similar to Fig. 1a. AC = apoptotic cell. *p < .05, **p < .01, ***p < .001. Data are representative of at least two independent experiments with 3–4 replicates per condition.
(b) Table presents the cycle numbers for each species-specific qPCR primer. None of these primers gave signals when tested against human Jurkat cell mRNA (target) alone.