Figure 2. Specific SLC signatures induced during different contexts of efferocytosis.
(a) SLC signature during efferocytosis is distinct from antibody-mediated phagocytosis. Peritoneal macrophages were incubated with apoptotic or anti-CD3 (IgG)-coated Jurkat cells, and qPCR of mouse SLC genes performed. Upregulated (green), downregulated (red), and unchanged (grey) are shown. (right) CypHer5E fluorescence within macrophages engulfing the targets. **p < .01, ***p < .001. Two independent experiments with 3–4 replicates per condition. (b) SLC modulation in efferocytic peritoneal macrophages in vivo. (left) Flow cytometric profiles of CD11bhighF4/80high and CD11blowF4/80low macrophages, and engulfing peritoneal macrophages (CypHer5e+). (right) qPCR using mouse-specific primers. **p < .01, ***p < .001. Data represent two replicates with 6 mice per group/experiment. (c) Specific SLC signature during different stages of efferocytosis. RNAseq was performed using mRNA from LR73 cells treated (4hr) with supernatants of apoptotic cells, or CytoD-treated LR73 cells incubated with apoptotic cells. SLC genes altered by supernatant alone (Smell), and CytoD-sensitive SLCs (Ingestion) were used to identify ligand:receptor responding SLCs (Taste) (red, upregulated; blue, downregulated). For clarity, SLCs in more than one stage are not shown.