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| Protocol | Endogenous label | IV-label | Stains | IHC | Time | Transparency |
|---|---|---|---|---|---|---|
| Optimized CUBIC | Preserved, GFP, RFP, collagen (SHG) | Preserved, rhodamine-lectin | Yes, DAPI, SYTO13, Streptavidin Alexa-Fluor 750 | Possible but slow | 3 days | Good |
| CUBIC | Preserved | Preserved | Yes | Possible But Slow | 5 days | Good |
| ScaleS | Preserved | Preserved | Yes | Possible but slow | 5 days | Moderate |